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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
4
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pubmed:dateCreated |
2004-8-25
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pubmed:abstractText |
Human osteoprotegrin (OPG) and its truncated mutant OPG-280 and lengthened mutant OPG-Fc were constructed and successfully expressed in Trichoplusia ni cells and Bombyx mori larvae. Native SDS-PAGE and Western blot analysis revealed that OPG-Fc is present as a homodimer in Tn cells or B. mori larvae compared with OPG and OPG-280. Furthermore, the hypocalcemic effect assay showed that truncation of the C-terminal 100 residues OPG does not abolish the biological activity and Fc can be helpful in forming the OPG homodimer with improved biological activity.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Calcium,
http://linkedlifedata.com/resource/pubmed/chemical/Glycoproteins,
http://linkedlifedata.com/resource/pubmed/chemical/Osteoprotegerin,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Cytoplasmic and Nuclear,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Tumor Necrosis Factor,
http://linkedlifedata.com/resource/pubmed/chemical/Recombinant Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/TNFRSF11B protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Tnfrsf11b protein, mouse
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pubmed:status |
MEDLINE
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pubmed:month |
Aug
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pubmed:issn |
0929-8665
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
11
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
317-23
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:15327363-Animals,
pubmed-meshheading:15327363-Bombyx,
pubmed-meshheading:15327363-Calcium,
pubmed-meshheading:15327363-Cells, Cultured,
pubmed-meshheading:15327363-Gene Expression,
pubmed-meshheading:15327363-Glycoproteins,
pubmed-meshheading:15327363-Humans,
pubmed-meshheading:15327363-Hypocalcemia,
pubmed-meshheading:15327363-Larva,
pubmed-meshheading:15327363-Lepidoptera,
pubmed-meshheading:15327363-Mice,
pubmed-meshheading:15327363-Mutation,
pubmed-meshheading:15327363-Osteoprotegerin,
pubmed-meshheading:15327363-Protein Structure, Quaternary,
pubmed-meshheading:15327363-Receptors, Cytoplasmic and Nuclear,
pubmed-meshheading:15327363-Receptors, Tumor Necrosis Factor,
pubmed-meshheading:15327363-Recombinant Proteins,
pubmed-meshheading:15327363-Transfection
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pubmed:year |
2004
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pubmed:articleTitle |
Production of recombinant human osteoprotegrin from Trichoplusia ni cells and Bombyx mori larvae.
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pubmed:affiliation |
State Key Laboratory of Molecular Biology, Shanghai Institute of Biochemistry And Cell Biology, Chinese Academy of Sciences, 320 Yueyang Road, Shanghai 200031, China.
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pubmed:publicationType |
Journal Article
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