Linked Life Data

1532580

Source:http://linkedlifedata.com/resource/pubmed/id/1532580

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
9
pubmed:dateCreated
1992-5-4
pubmed:abstractText
AUUUA multimers present in the 3'-untranslated region of mature lymphokine and cytokine transcripts have been implicated in the regulation of mRNA stability and translational efficiency. We have identified RNA-binding factors, termed AU-A, AU-B, and AU-C, that interact with AUUUA multimers. AU-A is an abundant, constitutively expressed 34-kDa factor that localizes primarily to the nucleus. AU-A binds to AUUUA multimers with low relative affinity and also binds to other U-rich sequences, including a poly(U) sequence. AU-B and AU-C are 30- and 43-kDa cytoplasmic factors that are induced following T cell receptor-mediated stimulation of purified human T cells and bind to AUUUA multimers with high affinity. Protease cleavage of AU-A, AU-B, and AU-C RNA-protein complexes indicate that AU-B and AU-C are structurally related to each other but distinct from AU-A. AU-B and AU-C require three or more tandem AUUUA repeats for efficient binding, and binding by these factors poorly tolerates mutations in the AUUUA recognition sequence. The precise binding specificity, high affinity, pattern of induction, and cytoplasmic localization all suggest that the structurally related AU-B and AU-C RNA-binding factors could be cytoplasmic regulators of lymphokine mRNA metabolism.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Mar
pubmed:issn
0021-9258
pubmed:author
pubmed:issnType
Print
pubmed:day
25
pubmed:volume
267
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
6302-9
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed-meshheading:1532580-Antigens, CD, pubmed-meshheading:1532580-Antigens, CD3, pubmed-meshheading:1532580-Antigens, Differentiation, T-Lymphocyte, pubmed-meshheading:1532580-Base Sequence, pubmed-meshheading:1532580-Cloning, Molecular, pubmed-meshheading:1532580-Cytoplasm, pubmed-meshheading:1532580-Humans, pubmed-meshheading:1532580-Molecular Sequence Data, pubmed-meshheading:1532580-Molecular Weight, pubmed-meshheading:1532580-Oligodeoxyribonucleotides, pubmed-meshheading:1532580-Plasmids, pubmed-meshheading:1532580-RNA-Binding Proteins, pubmed-meshheading:1532580-Receptors, Antigen, T-Cell, pubmed-meshheading:1532580-Repetitive Sequences, Nucleic Acid, pubmed-meshheading:1532580-Substrate Specificity, pubmed-meshheading:1532580-T-Lymphocytes, pubmed-meshheading:1532580-Transcription, Genetic
pubmed:year
1992
pubmed:articleTitle
AU RNA-binding factors differ in their binding specificities and affinities.
pubmed:affiliation
Cellular and Molecular Biology Program, University of Michigan, Ann Arbor 48109.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't