Linked Life Data

15323417

Source:http://linkedlifedata.com/resource/pubmed/id/15323417

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
2004-8-24
pubmed:abstractText
The protoplast fusion was induced between Astragalus adsurgens Pall and Agrobacterium rhizogenes-transformed cell line of Medicago Sativa L. by use of PEG method. Putative somatic hybrid cells were selected based on the observation that only intergeneric hybrid cells continued to divide on the DPD medium without any phytohormone while the cells and clusters derived from parental cells did not survive further. After being cultured, the somatic hybrid calli were obtained. Although both of the parental calli were not capable of regenerating , one of the hydrid cell lines recovered the differentiation ability and produced small shoots. Characterizations of the hybrid calli were carried out by examination of chromsome number, opine synthetase assay, isoenzyme and RAPD analyses.
pubmed:language
chi
pubmed:journal
pubmed:citationSubset
IM
pubmed:status
MEDLINE
pubmed:month
Jun
pubmed:issn
0001-5334
pubmed:author
pubmed:issnType
Print
pubmed:volume
37
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
167-75
pubmed:meshHeading
pubmed:year
2004
pubmed:articleTitle
[Intergeneric somatic hybridization between Astragalus adsurgens Pall and Medicago sativa L].
pubmed:affiliation
Provincial Key Laboratory of Biotechnology, Northwest University, Xi'an 710069.
pubmed:publicationType
Journal Article, English Abstract, Research Support, Non-U.S. Gov't