Linked Life Data

15322810

Source:http://linkedlifedata.com/resource/pubmed/id/15322810

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
7
pubmed:dateCreated
2005-10-17
pubmed:abstractText
Cultured cells of tobacco (Nicotiana tabacum L. cv Petit Havana) were used to investigate signals regulating the expression of the "model" nuclear gene encoding the alternative oxidase (AOX) (AOX1), the terminal oxidase of the mitochondrial alternative respiratory pathway. Several conditions shown to induce AOX1 mRNA accumulation also result in an increase in cellular citrate concentrations, suggesting that citrate and/or other tricarboxylic acid (TCA) cycle intermediates may be important signal metabolites. In addition, mitochondrial reactive oxygen species (ROS) production has recently been shown to be a factor mediating mitochondria-to-nucleus signaling for the expression of AOX1. We found that the exogenously supplied TCA cycle organic acids citrate, malate and 2-oxoglutarate caused rapid and dramatic increases in the steady-state level of AOX1 mRNA at low, near physiological concentrations (0.1 mM). Furthermore, an increase in AOX1 induced by the addition of organic acids occurs independently of mitochondrial ROS formation. Our results demonstrate that two separate pathways for mitochondria-to-nucleus signaling of AOX1 may exist, one involving ROS and the other organic acids.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Dec
pubmed:issn
0721-7714
pubmed:author
pubmed:issnType
Print
pubmed:volume
23
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
497-503
pubmed:dateRevised
2011-11-17
pubmed:meshHeading
pubmed-meshheading:15322810-Cell Nucleus, pubmed-meshheading:15322810-Cells, Cultured, pubmed-meshheading:15322810-Citric Acid Cycle, pubmed-meshheading:15322810-Energy Metabolism, pubmed-meshheading:15322810-Gene Expression Regulation, Enzymologic, pubmed-meshheading:15322810-Gene Expression Regulation, Plant, pubmed-meshheading:15322810-Mitochondria, pubmed-meshheading:15322810-Mitochondrial Proteins, pubmed-meshheading:15322810-Oxidative Stress, pubmed-meshheading:15322810-Oxidoreductases, pubmed-meshheading:15322810-Plant Proteins, pubmed-meshheading:15322810-RNA, Messenger, pubmed-meshheading:15322810-Reactive Oxygen Species, pubmed-meshheading:15322810-Signal Transduction, pubmed-meshheading:15322810-Tobacco, pubmed-meshheading:15322810-Tricarboxylic Acids, pubmed-meshheading:15322810-Up-Regulation
pubmed:year
2004
pubmed:articleTitle
Mitochondria/nuclear signaling of alternative oxidase gene expression occurs through distinct pathways involving organic acids and reactive oxygen species.
pubmed:affiliation
Department of Plant Sciences, University of Saskatchewan, Saskatoon, SK, Canada, S7N 5A8. gr.gray@usask.ca
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, Non-P.H.S., Research Support, Non-U.S. Gov't