Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
1992-4-22
pubmed:abstractText
Recent studies have identified a conserved 28-bp element (HF-1) within the rat cardiac MLC-2 gene which confers cardiac muscle-specific and inducible expression during myocardial cell hypertrophy. Utilizing a combination of independent experimental approaches, this study characterizes two cardiac nuclear factors which bind to HF-1, a ubiquitous factor (HF-1a), and an A + T-rich binding factor (HF-1b) which is preferentially expressed in differentiated cardiac and skeletal muscle cells. The HF-1a binding site is located in a core region of the 28-bp conserved element, immediately upstream from the A + T-rich HF-1b site, which is homologous to the MEF-2 site found in a number of muscle genes. By a number of separate criteria (gel mobility shift, competition, and mutagenesis studies), HF-1b and MEF-2 appear to be indistinguishable and thus are either identical or closely related muscle factors. Transient assays of luciferase reporter genes containing point mutations throughout the 28-bp HF-1 regulatory element document the importance of both the HF-1a and HF-1b sites in transient assays in ventricular muscle cells. In the native 250-bp MLC-2 promoter fragment, mutations in the single E box had little effect on cardiac muscle specificity, while point mutations in either the HF-1a or HF-1b binding site significantly reduced promoter activity, underscoring the importance of both the HF-1a and HF-1b sites in the transcriptional activation of this cardiac muscle gene. Thus, this study provides evidence that a novel, ubiquitous factor (HF-1a) and a muscle factor (HF-1b/MEF-2) can form a novel, E-box-independent pathway for muscle-specific expression in ventricular cardiac muscle cells.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/1532229-1649701, http://linkedlifedata.com/resource/pubmed/commentcorrection/1532229-1656214, http://linkedlifedata.com/resource/pubmed/commentcorrection/1532229-1673020, http://linkedlifedata.com/resource/pubmed/commentcorrection/1532229-1690844, http://linkedlifedata.com/resource/pubmed/commentcorrection/1532229-1694017, http://linkedlifedata.com/resource/pubmed/commentcorrection/1532229-1696258, http://linkedlifedata.com/resource/pubmed/commentcorrection/1532229-1835945, http://linkedlifedata.com/resource/pubmed/commentcorrection/1532229-1846704, http://linkedlifedata.com/resource/pubmed/commentcorrection/1532229-1848675, http://linkedlifedata.com/resource/pubmed/commentcorrection/1532229-1989904, http://linkedlifedata.com/resource/pubmed/commentcorrection/1532229-1993702, http://linkedlifedata.com/resource/pubmed/commentcorrection/1532229-1996097, http://linkedlifedata.com/resource/pubmed/commentcorrection/1532229-2065979, http://linkedlifedata.com/resource/pubmed/commentcorrection/1532229-2115126, http://linkedlifedata.com/resource/pubmed/commentcorrection/1532229-2123467, http://linkedlifedata.com/resource/pubmed/commentcorrection/1532229-2154748, http://linkedlifedata.com/resource/pubmed/commentcorrection/1532229-2156629, http://linkedlifedata.com/resource/pubmed/commentcorrection/1532229-2171781, http://linkedlifedata.com/resource/pubmed/commentcorrection/1532229-2174572, http://linkedlifedata.com/resource/pubmed/commentcorrection/1532229-2200736, http://linkedlifedata.com/resource/pubmed/commentcorrection/1532229-2253873, http://linkedlifedata.com/resource/pubmed/commentcorrection/1532229-2300571, http://linkedlifedata.com/resource/pubmed/commentcorrection/1532229-2311584, http://linkedlifedata.com/resource/pubmed/commentcorrection/1532229-2370866, http://linkedlifedata.com/resource/pubmed/commentcorrection/1532229-2493990, http://linkedlifedata.com/resource/pubmed/commentcorrection/1532229-2503252, http://linkedlifedata.com/resource/pubmed/commentcorrection/1532229-2536844, http://linkedlifedata.com/resource/pubmed/commentcorrection/1532229-2537150, http://linkedlifedata.com/resource/pubmed/commentcorrection/1532229-2550138, http://linkedlifedata.com/resource/pubmed/commentcorrection/1532229-2552320, http://linkedlifedata.com/resource/pubmed/commentcorrection/1532229-2560751, http://linkedlifedata.com/resource/pubmed/commentcorrection/1532229-2601707, http://linkedlifedata.com/resource/pubmed/commentcorrection/1532229-2721498, http://linkedlifedata.com/resource/pubmed/commentcorrection/1532229-2761542, http://linkedlifedata.com/resource/pubmed/commentcorrection/1532229-2777078, http://linkedlifedata.com/resource/pubmed/commentcorrection/1532229-2788278, http://linkedlifedata.com/resource/pubmed/commentcorrection/1532229-2796988, http://linkedlifedata.com/resource/pubmed/commentcorrection/1532229-2808370, http://linkedlifedata.com/resource/pubmed/commentcorrection/1532229-2822255, http://linkedlifedata.com/resource/pubmed/commentcorrection/1532229-2828167, http://linkedlifedata.com/resource/pubmed/commentcorrection/1532229-2830491, http://linkedlifedata.com/resource/pubmed/commentcorrection/1532229-2833748, http://linkedlifedata.com/resource/pubmed/commentcorrection/1532229-2835372, http://linkedlifedata.com/resource/pubmed/commentcorrection/1532229-2889129, http://linkedlifedata.com/resource/pubmed/commentcorrection/1532229-2964082, http://linkedlifedata.com/resource/pubmed/commentcorrection/1532229-3047142, http://linkedlifedata.com/resource/pubmed/commentcorrection/1532229-3198615, http://linkedlifedata.com/resource/pubmed/commentcorrection/1532229-3240859, http://linkedlifedata.com/resource/pubmed/commentcorrection/1532229-3380697, http://linkedlifedata.com/resource/pubmed/commentcorrection/1532229-3413104, http://linkedlifedata.com/resource/pubmed/commentcorrection/1532229-3524858, http://linkedlifedata.com/resource/pubmed/commentcorrection/1532229-3670292, http://linkedlifedata.com/resource/pubmed/commentcorrection/1532229-3690668, http://linkedlifedata.com/resource/pubmed/commentcorrection/1532229-3785189, http://linkedlifedata.com/resource/pubmed/commentcorrection/1532229-3785201, http://linkedlifedata.com/resource/pubmed/commentcorrection/1532229-3821727, http://linkedlifedata.com/resource/pubmed/commentcorrection/1532229-6828386
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Apr
pubmed:issn
0270-7306
pubmed:author
pubmed:issnType
Print
pubmed:volume
12
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1469-79
pubmed:dateRevised
2010-9-7
pubmed:meshHeading
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