Source:http://linkedlifedata.com/resource/pubmed/id/15319543
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
4-6
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pubmed:dateCreated |
2004-8-20
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pubmed:abstractText |
Effect of epinephrine on alpha-methyl-D-glucopyranoside uptake in renal proximal tubule cells. Epinephrine has known to be a very important factor in the regulation of renal sodium excretion. However, the effect of epinephrine on Na+/glucose cotransporter was not fully elucidated. Thus, we examined effect of epinephrine on alpha-methyl-D-glucopyranoside (alpha-MG) uptake and its related signal pathways in the primary cultured rabbit renal proximal tubule cells (PTCs). Epinephrine inhibited alpha-MG uptake in a time- and dose-dependent manner and also decreased SGLT1 and SGLT2 protein level. Both phentolamine and propranolol completely prevented epinephrine-induced inhibition of alpha-MG uptake. The epinephrine-induced inhibition of alpha-MG uptake was blocked by SQ-22536 or myristoylated PKA inhibitor amide 14-22 and epinephrine increased the intracellular cAMP content. In western blotting analysis, epinephrine increases phosphorylation of p44/42 and p38 MAPKs and PD 98059 or SB 203580 blocked the effect of epinephrine. In addition, epinephrine increased AA release and PGE2 production and effects of epinephrine on alpha-MG uptake and AA release were blocked by staurosporine and bisindolylmaleimide I or mepacrine and AACOCF3. Indeed, epinephrine translocated PKC or cPLA2 from cytosol to membrane fraction. In conclusion, epinephrine partially inhibits the alpha-MG uptake through PKA, PKC, p44/42, p38 MAPK, and cPLA2 pathways in the PTCs.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Epinephrine,
http://linkedlifedata.com/resource/pubmed/chemical/Methylglucosides,
http://linkedlifedata.com/resource/pubmed/chemical/Monosaccharide Transport Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Phentolamine,
http://linkedlifedata.com/resource/pubmed/chemical/Propranolol,
http://linkedlifedata.com/resource/pubmed/chemical/Protein-Serine-Threonine Kinases,
http://linkedlifedata.com/resource/pubmed/chemical/SLC5A2 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Sodium-Glucose Transporter 2,
http://linkedlifedata.com/resource/pubmed/chemical/methylglucoside
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pubmed:status |
MEDLINE
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pubmed:issn |
1015-8987
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pubmed:author | |
pubmed:copyrightInfo |
Copyright 2004 S. Karger AG, Basel
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pubmed:issnType |
Print
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pubmed:volume |
14
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
395-406
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pubmed:dateRevised |
2007-11-15
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pubmed:meshHeading |
pubmed-meshheading:15319543-Animals,
pubmed-meshheading:15319543-Biological Transport,
pubmed-meshheading:15319543-Cells, Cultured,
pubmed-meshheading:15319543-Epinephrine,
pubmed-meshheading:15319543-Kidney Tubules, Proximal,
pubmed-meshheading:15319543-Methylglucosides,
pubmed-meshheading:15319543-Monosaccharide Transport Proteins,
pubmed-meshheading:15319543-Phentolamine,
pubmed-meshheading:15319543-Propranolol,
pubmed-meshheading:15319543-Protein-Serine-Threonine Kinases,
pubmed-meshheading:15319543-Rats,
pubmed-meshheading:15319543-Sodium-Glucose Transporter 2
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pubmed:year |
2004
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pubmed:articleTitle |
Effect of epinephrine on alpha-methyl-D-glucopyranoside uptake in renal proximal tubule cells.
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pubmed:affiliation |
Department of Veterinary Physiology, College of Veterinary Medicine, Chonnam National University, Gwangju, Korea.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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