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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
6
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pubmed:dateCreated |
1992-4-9
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pubmed:databankReference | |
pubmed:abstractText |
TCR V beta promoter contains a highly conserved decamer homologous to cAMP response element (CRE). Recent studies have identified this CRE decamer as the dominant transcription-activating element within the TCR V beta promoter. We have isolated cDNA clones, TCR-ATF1 and TCR-ATF2, encoding DNA-binding proteins that recognize this CRE motif. The nucleotide sequence of TCR-ATF1 has not previously been reported, whereas that of TCR-ATF2 was homologous to CRE-BP1, ATF-2, and mXBP. Both TCR-ATF1 and TCR-ATF2 shared a conserved leucine zipper and DNA binding motif with other CRE-binding proteins. TCR-ATF1 and TCR-ATF2 were expressed in all cell lines examined and in mouse embryos as early as 12.5 days. Despite binding to the same CRE motif, TCR-ATF1 and TCR-ATF2 were different from CREB in the fine nucleotide specificity. TCR-ATF bound methylated CRE and CRE mutant M4 (4C----G) that were not recognized by CREB. Additionally, TCR-ATF1 weakly recognized two other single nucleotide mutants of V beta-CRE that were not bound by TCR-ATF2 and CREB. We have further demonstrated that TCR beta-chain expression was immediately activated by cAMP. Such induction is likely mediated through V beta-CRE sequence, because the inclusion of V beta-CRE in a vector with minimum promoter (pBLCAT2) conferred the cAMP inducibility of CAT activity.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
AIM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Cyclic AMP Response...,
http://linkedlifedata.com/resource/pubmed/chemical/DNA-Binding Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Nuclear Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Oligodeoxyribonucleotides,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Antigen, T-Cell...,
http://linkedlifedata.com/resource/pubmed/chemical/Transcription Factors
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pubmed:status |
MEDLINE
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pubmed:month |
Mar
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pubmed:issn |
0022-1767
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:day |
15
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pubmed:volume |
148
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1906-12
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pubmed:dateRevised |
2008-11-21
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pubmed:meshHeading |
pubmed-meshheading:1531847-Amino Acid Sequence,
pubmed-meshheading:1531847-Animals,
pubmed-meshheading:1531847-Base Sequence,
pubmed-meshheading:1531847-Cloning, Molecular,
pubmed-meshheading:1531847-Cyclic AMP Response Element-Binding Protein,
pubmed-meshheading:1531847-DNA-Binding Proteins,
pubmed-meshheading:1531847-Gene Expression,
pubmed-meshheading:1531847-Mice,
pubmed-meshheading:1531847-Molecular Sequence Data,
pubmed-meshheading:1531847-Nuclear Proteins,
pubmed-meshheading:1531847-Oligodeoxyribonucleotides,
pubmed-meshheading:1531847-Promoter Regions, Genetic,
pubmed-meshheading:1531847-Protein Binding,
pubmed-meshheading:1531847-Receptors, Antigen, T-Cell, alpha-beta,
pubmed-meshheading:1531847-Regulatory Sequences, Nucleic Acid,
pubmed-meshheading:1531847-Transcription Factors
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pubmed:year |
1992
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pubmed:articleTitle |
Isolation and characterization of nuclear proteins that bind to T cell receptor V beta decamer motif.
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pubmed:affiliation |
Institute of Molecular Biology, Academia Sinica, Taipei, Taiwan, R.O.C.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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