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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
35
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pubmed:dateCreated |
2004-9-1
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pubmed:abstractText |
CCAAT enhancer-binding protein beta (C/EBPbeta), a basic-leucine zipper transcription factor, is an important effector of signals in physiologic growth and cancer. The identification of direct C/EBPbeta targets in vivo has been limited by functional compensation by other C/EBP family proteins and the low stringency of the consensus sequence. Here we use the combined power of expression profiling and high-throughput chromatin immunoprecipitation to identify direct and biologically relevant targets of C/EBPbeta. We identified 25 potential C/EBPbeta targets, of which 88% of those tested were confirmed as in vivo C/EBPbeta-binding sites. Six of these genes also displayed differential expression in C/EBPbeta-/- livers. Computational analysis revealed that bona fide C/EBPbeta target genes can be distinguished by the presence of binding motifs for specific additional transcription factors in the vicinity of the C/EBPbeta site. This approach is generally applicable to the discovery of direct, biologically relevant targets of mammalian transcription factors.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/15317935-10085237,
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http://linkedlifedata.com/resource/pubmed/commentcorrection/15317935-8632009,
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http://linkedlifedata.com/resource/pubmed/commentcorrection/15317935-8997490,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15317935-9322048,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15317935-9334201,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15317935-9414177,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15317935-9426001,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15317935-9727068,
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http://linkedlifedata.com/resource/pubmed/commentcorrection/15317935-9792624,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15317935-9822619
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Aug
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pubmed:issn |
0027-8424
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pubmed:author |
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pubmed:copyrightInfo |
Copyright 2004 The National Academy of Sciencs of the USA
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pubmed:issnType |
Print
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pubmed:day |
31
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pubmed:volume |
101
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
12986-91
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
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pubmed:year |
2004
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pubmed:articleTitle |
Orthogonal analysis of C/EBPbeta targets in vivo during liver proliferation.
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pubmed:affiliation |
Department of Genetics, Bioinformatics Core, University of Pennsylvania School of Medicine, Philadelphia, PA 19104, USA.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
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