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Predicate | Object |
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rdf:type | |
lifeskim:mentions |
umls-concept:C0021756,
umls-concept:C0021761,
umls-concept:C0085862,
umls-concept:C0086418,
umls-concept:C0205251,
umls-concept:C0205263,
umls-concept:C0814999,
umls-concept:C1299583,
umls-concept:C1514485,
umls-concept:C1549571,
umls-concept:C1608386,
umls-concept:C1704259,
umls-concept:C1705987,
umls-concept:C1709634
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pubmed:issue |
1
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pubmed:dateCreated |
1992-4-3
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pubmed:abstractText |
The proliferation potential of highly purified human CD3-CD4-CD8- (triple negative) and CD3low(lo)CD4-CD8- thymocyte precursors in response to various cytokines was investigated. High in vitro growth ability was observed in response to recombinant human IL-2 (rIL-2) and human rIL-7, both in the absence of any co-mitogen and in combination with phorbol 12-myristate 13-acetate (PMA). Furthermore, the proliferation of these thymocyte precursors in the presence of rIL-7, although accompanied by a significant increase of IL-2 receptor (IL-2R) p55 expression, appeared independent of that mediated by the autocrine IL-2 pathway, since mAbs to IL-2 and IL-2R p55 did not eliminate responsiveness to rIL-7. Synergism of rIL-7 with rIL-2 was also observed, while no cooperation was detectable with rIL-4 or rIL-6. Analysis of surface phenotype and cell cycle status of cells cultured in the presence of rIL-7, both plus and minus PMA, showed that CD3- as well as CD3lo cells readily proliferated to rIL-7. Upregulation of the levels of expression of CD3 antigen was also observed in these cultures. These results, together with the previous characterization of IL-7 as a human pre-B cell and mature T cell growth factor, identify IL-7 as a cytokine with biologic activities on a variety of target cells. They also suggest that IL-7, in analogy with the mouse system, might play a role in human T cell ontogeny.
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pubmed:grant | |
pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD3,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD4,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD8,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, Differentiation...,
http://linkedlifedata.com/resource/pubmed/chemical/Interleukin-2,
http://linkedlifedata.com/resource/pubmed/chemical/Interleukin-7,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Antigen, T-Cell
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pubmed:status |
MEDLINE
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pubmed:month |
Jan
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pubmed:issn |
0953-8178
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
4
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1-5
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pubmed:dateRevised |
2007-11-14
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pubmed:meshHeading |
pubmed-meshheading:1531763-Antigens, CD3,
pubmed-meshheading:1531763-Antigens, CD4,
pubmed-meshheading:1531763-Antigens, CD8,
pubmed-meshheading:1531763-Antigens, Differentiation, T-Lymphocyte,
pubmed-meshheading:1531763-Cell Division,
pubmed-meshheading:1531763-Child,
pubmed-meshheading:1531763-Hematopoietic Stem Cells,
pubmed-meshheading:1531763-Humans,
pubmed-meshheading:1531763-Interleukin-2,
pubmed-meshheading:1531763-Interleukin-7,
pubmed-meshheading:1531763-Receptors, Antigen, T-Cell,
pubmed-meshheading:1531763-T-Lymphocyte Subsets
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pubmed:year |
1992
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pubmed:articleTitle |
IL-7 induces proliferation of CD3-/low CD4- CD8- human thymocyte precursors by an IL-2 independent pathway.
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pubmed:affiliation |
Department of Biochemistry and Molecular Biology, Harvard University, Cambridge, MA 02138.
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pubmed:publicationType |
Journal Article,
In Vitro,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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