Linked Life Data

15315859

Source:http://linkedlifedata.com/resource/pubmed/id/15315859

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
27-28
pubmed:dateCreated
2004-8-18
pubmed:abstractText
Cynomolgus macaques were immunized by either the intramuscular (i.m.) or intranasal (i.n.) route with a HIV-1 peptide-based immunogen (C4-V3 89.6P) alone, or formulated with novel adjuvants to evaluate the ability of the adjuvants to augment peptide-specific systemic and mucosal immune responses. A mutant cholera toxin, CT-E29H, or the combination of recombinant human IL-1alpha (rhIL-1alpha) protein and recombinant human GM-CSF (rhGM-CSF) protein were tested as adjuvants for i.n. immunization, while a stable emulsion of a synthetic monophosphoryl lipid A (MPL) analogue (RC529-SE) plus rhGM-CSF protein was tested as an adjuvant for i.m. immunization. Macaques immunized i.n. with peptide alone failed to elicit an anti-C4-V3 89.6P antibody response in serum. In contrast, all the tested peptide/adjuvant formulations elicited peptide-specific immune responses. RC529-SE/rhGM-CSF elicited the highest peak anti-peptide IgG geometric mean titer in serum (1:32,768 at week 25) followed by rhIL-1alpha/rhGM-CSF (1:1217 at week 10) and CT-E29H (1:256 at week 25). Measurable SHIV neutralizing antibody responses were detectable in only one macaque immunized i.m. with peptide formulated with RC529-SE/rhGM-CSF. Macaques immunized by the i.n. route with peptide in combination with CT-E29H failed to elicit measurable antibody responses at nasal or genital mucosal surfaces. In contrast, antibody responses at the nasal and genital mucosa were detected in macaques immunized by the i.n. route with peptide in combination with rhIL-1alpha/rhGM-CSF. However, antibody responses at the nasal and genital mucosa were highest in macaques immunized parenterally with peptide in combination with the adjuvants RC529-SE/rhGM-CSF. These results suggest that parenteral vaccine administration in combination with the appropriate adjuvant formulation can elicit vaccine-specific humoral immune responses in both systemic and mucosal compartments.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Sep
pubmed:issn
0264-410X
pubmed:author
pubmed:issnType
Print
pubmed:day
9
pubmed:volume
22
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
3774-88
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed-meshheading:15315859-AIDS Vaccines, pubmed-meshheading:15315859-Adjuvants, Immunologic, pubmed-meshheading:15315859-Administration, Intranasal, pubmed-meshheading:15315859-Amino Acid Sequence, pubmed-meshheading:15315859-Animals, pubmed-meshheading:15315859-Antibodies, Bacterial, pubmed-meshheading:15315859-Antibody Formation, pubmed-meshheading:15315859-Antibody Specificity, pubmed-meshheading:15315859-Cholera Toxin, pubmed-meshheading:15315859-Dose-Response Relationship, Immunologic, pubmed-meshheading:15315859-Enzyme-Linked Immunosorbent Assay, pubmed-meshheading:15315859-Female, pubmed-meshheading:15315859-Granulocyte-Macrophage Colony-Stimulating Factor, pubmed-meshheading:15315859-HIV-1, pubmed-meshheading:15315859-Immunization Schedule, pubmed-meshheading:15315859-Immunoglobulin A, pubmed-meshheading:15315859-Immunoglobulin G, pubmed-meshheading:15315859-Immunoglobulins, pubmed-meshheading:15315859-Macaca fascicularis, pubmed-meshheading:15315859-Molecular Sequence Data, pubmed-meshheading:15315859-Neutralization Tests, pubmed-meshheading:15315859-Simian immunodeficiency virus, pubmed-meshheading:15315859-Vaccines, Subunit
pubmed:year
2004
pubmed:articleTitle
A comparative evaluation of nasal and parenteral vaccine adjuvants to elicit systemic and mucosal HIV-1 peptide-specific humoral immune responses in cynomolgus macaques.
pubmed:affiliation
Wyeth Vaccines Research, Pearl River, NY 10965, USA.
pubmed:publicationType
Journal Article, Comparative Study