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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
4
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pubmed:dateCreated |
2004-8-18
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pubmed:abstractText |
Initiation of meiotic recombination by double-strand breaks (DSBs) must occur in a controlled fashion to avoid jeopardizing genome integrity. Here, we identify chromatin-associated protein HIM-17 as a link between chromatin state and DSB formation during C. elegans meiosis. Dependencies of several meiotic prophase events on HIM-17 parallel those seen for DSB-generating enzyme SPO-11: HIM-17 is essential for DSB formation but dispensable for homolog synapsis. Crossovers and chiasmata are eliminated in him-17 null mutants but are restored by artificially induced DSBs, indicating that all components required to convert DSBs into chiasmata are present. Unlike SPO-11, HIM-17 is also required for proper accumulation of histone H3 methylation at lysine 9 on meiotic prophase chromosomes. HIM-17 shares structural features with three proteins that interact genetically with LIN-35/Rb, a known component of chromatin-modifying complexes. Furthermore, DSB levels and incidence of chiasmata can be modulated by loss of LIN-35/Rb. These and other data suggest that chromatin state governs the timing of DSB competence.
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pubmed:grant |
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pubmed:commentsCorrections |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Caenorhabditis elegans Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Cell Cycle Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Chromatin,
http://linkedlifedata.com/resource/pubmed/chemical/DAPI,
http://linkedlifedata.com/resource/pubmed/chemical/DNA-Binding Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Endodeoxyribonucleases,
http://linkedlifedata.com/resource/pubmed/chemical/Esterases,
http://linkedlifedata.com/resource/pubmed/chemical/Green Fluorescent Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Histones,
http://linkedlifedata.com/resource/pubmed/chemical/Indoles,
http://linkedlifedata.com/resource/pubmed/chemical/Luminescent Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Lysine,
http://linkedlifedata.com/resource/pubmed/chemical/Rad51 Recombinase,
http://linkedlifedata.com/resource/pubmed/chemical/meiotic recombination protein SPO11,
http://linkedlifedata.com/resource/pubmed/chemical/rad-51 protein, C elegans
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pubmed:status |
MEDLINE
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pubmed:month |
Aug
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pubmed:issn |
0092-8674
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
20
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pubmed:volume |
118
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
439-52
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pubmed:dateRevised |
2010-11-18
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pubmed:meshHeading |
pubmed-meshheading:15315757-Alleles,
pubmed-meshheading:15315757-Amino Acid Sequence,
pubmed-meshheading:15315757-Animals,
pubmed-meshheading:15315757-Caenorhabditis elegans,
pubmed-meshheading:15315757-Caenorhabditis elegans Proteins,
pubmed-meshheading:15315757-Cell Cycle Proteins,
pubmed-meshheading:15315757-Chromatin,
pubmed-meshheading:15315757-Chromosome Mapping,
pubmed-meshheading:15315757-DNA Methylation,
pubmed-meshheading:15315757-DNA-Binding Proteins,
pubmed-meshheading:15315757-Endodeoxyribonucleases,
pubmed-meshheading:15315757-Esterases,
pubmed-meshheading:15315757-Green Fluorescent Proteins,
pubmed-meshheading:15315757-Histones,
pubmed-meshheading:15315757-In Situ Hybridization, Fluorescence,
pubmed-meshheading:15315757-Indoles,
pubmed-meshheading:15315757-Karyotyping,
pubmed-meshheading:15315757-Luminescent Proteins,
pubmed-meshheading:15315757-Lysine,
pubmed-meshheading:15315757-Meiosis,
pubmed-meshheading:15315757-Models, Genetic,
pubmed-meshheading:15315757-Molecular Sequence Data,
pubmed-meshheading:15315757-Mutation,
pubmed-meshheading:15315757-Phenotype,
pubmed-meshheading:15315757-Prophase,
pubmed-meshheading:15315757-Protein Structure, Tertiary,
pubmed-meshheading:15315757-RNA Interference,
pubmed-meshheading:15315757-Rad51 Recombinase,
pubmed-meshheading:15315757-Recombination, Genetic,
pubmed-meshheading:15315757-Sequence Homology, Amino Acid,
pubmed-meshheading:15315757-Time Factors
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pubmed:year |
2004
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pubmed:articleTitle |
C. elegans HIM-17 links chromatin modification and competence for initiation of meiotic recombination.
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pubmed:affiliation |
Department of Developmental Biology, Stanford University School of Medicine, Stanford, CA 94305, USA.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, U.S. Gov't, Non-P.H.S.,
Research Support, Non-U.S. Gov't
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