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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
2
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pubmed:dateCreated |
1992-3-16
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pubmed:abstractText |
Recent experiments have shown that the E2F transcription factor is in a complex with the RB1 gene product. The E2F-pRB complex can be reconstituted in an in vitro assay using a GST-RB fusion protein isolated from Escherichia coli. This interaction is dependent on pRB sequences involved in E1A/T-antigen binding as well as carboxy-terminal pRB sequences that are not necessary for E1A/T binding. Moreover, reconstitution assays reveal a requirement for an accessory factor, in addition to E2F and pRB, for formation of the E2F-pRB complex. Assays of transcription from the adenovirus E2 promoter in transfection experiments demonstrate that formation of the complex containing pRB and E2F coincides with an inhibition of E2F-dependent transcriptional activity. A mutant pRB protein that does not associate with E2F does not inhibit transcription. We conclude that as a consequence of its interaction with E2F, pRB may regulate the transcriptional function of the E2F factor.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Adenovirus Early Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, Viral, Tumor,
http://linkedlifedata.com/resource/pubmed/chemical/Carrier Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Cell Cycle Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Chloramphenicol O-Acetyltransferase,
http://linkedlifedata.com/resource/pubmed/chemical/DNA-Binding Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/E2F Transcription Factors,
http://linkedlifedata.com/resource/pubmed/chemical/Oncogene Proteins, Viral,
http://linkedlifedata.com/resource/pubmed/chemical/Recombinant Fusion Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Retinoblastoma Protein,
http://linkedlifedata.com/resource/pubmed/chemical/Retinoblastoma-Binding Protein 1,
http://linkedlifedata.com/resource/pubmed/chemical/Transcription Factor DP1,
http://linkedlifedata.com/resource/pubmed/chemical/Transcription Factors
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pubmed:status |
MEDLINE
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pubmed:month |
Feb
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pubmed:issn |
0890-9369
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
6
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pubmed:geneSymbol |
RB1
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
177-85
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pubmed:dateRevised |
2009-11-19
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pubmed:meshHeading |
pubmed-meshheading:1531329-Adenovirus Early Proteins,
pubmed-meshheading:1531329-Antigens, Viral, Tumor,
pubmed-meshheading:1531329-Carrier Proteins,
pubmed-meshheading:1531329-Cell Cycle Proteins,
pubmed-meshheading:1531329-Chloramphenicol O-Acetyltransferase,
pubmed-meshheading:1531329-DNA-Binding Proteins,
pubmed-meshheading:1531329-E2F Transcription Factors,
pubmed-meshheading:1531329-Genes, Retinoblastoma,
pubmed-meshheading:1531329-HeLa Cells,
pubmed-meshheading:1531329-Humans,
pubmed-meshheading:1531329-Mutation,
pubmed-meshheading:1531329-Oncogene Proteins, Viral,
pubmed-meshheading:1531329-Precipitin Tests,
pubmed-meshheading:1531329-Promoter Regions, Genetic,
pubmed-meshheading:1531329-Recombinant Fusion Proteins,
pubmed-meshheading:1531329-Retinoblastoma Protein,
pubmed-meshheading:1531329-Retinoblastoma-Binding Protein 1,
pubmed-meshheading:1531329-Transcription, Genetic,
pubmed-meshheading:1531329-Transcription Factor DP1,
pubmed-meshheading:1531329-Transcription Factors
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pubmed:year |
1992
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pubmed:articleTitle |
The interaction of RB with E2F coincides with an inhibition of the transcriptional activity of E2F.
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pubmed:affiliation |
Howard Hughes Medical Institute, Duke University Medical Center, Durham, North Carolina 27710.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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