|
rdf:type |
|
|
lifeskim:mentions |
|
|
pubmed:issue |
43
|
|
pubmed:dateCreated |
2004-10-18
|
|
pubmed:abstractText |
The interferon regulatory factors (IRF) are transcriptional mediators of cellular response to viral invasion that play a critical role in the innate antiviral defense. Two of these factors, IRF-5 and IRF-7, play a critical role in the induction of interferon (IFNA) genes in infected cells; they are expressed constitutively in monocytes, B cells, and precursors of dendritic cells (pDC2) that are high producers of interferon alpha, and their expression can be further stimulated by type I interferon. The goal of the present study was to identify and analyze expression of cellular genes that are modulated by IRF-5 and IRF-7 during the innate response to viral infection. The transcription profiles of infected BJAB cells overexpressing IRF-5 or IRF-7 were determined by using oligonucleotide arrays with probe sets representing about 6800 human genes. This analysis shows that IRF-5 and IRF-7 activate a broad profile of heterologous genes encoding not only antiviral, inflammatory, and pro-apoptotic proteins but also proteins of other functional categories. The number of IRF-5- and IRF-7-modulated genes was significantly higher in infected than in uninfected cells, and the transcription signature was predominantly positive. Although IRF-5 and IRF-7 stimulated a large number of common genes, a distinct functional profile was associated with each of these IRFs. The noted difference was a broad antiviral and early inflammatory transcriptional profile in infected BJAB/IRF-5 cells, whereas the IRF-7-induced transcripts were enriched for the group of mitochondrial genes and genes affecting the DNA structure. Taken together, these data indicate that IRF-5 and IRF-7 act primarily as transcriptional activators and that IRF-5-and IRF-7-induced innate antiviral response results in a broad alteration of the transcriptional profile of cellular genes.
|
|
pubmed:grant |
|
|
pubmed:language |
eng
|
|
pubmed:journal |
|
|
pubmed:citationSubset |
IM
|
|
pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/CXCL11 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Carbon Tetrachloride,
http://linkedlifedata.com/resource/pubmed/chemical/Chemokine CXCL11,
http://linkedlifedata.com/resource/pubmed/chemical/Chemokines, CXC,
http://linkedlifedata.com/resource/pubmed/chemical/DNA,
http://linkedlifedata.com/resource/pubmed/chemical/DNA, Complementary,
http://linkedlifedata.com/resource/pubmed/chemical/DNA-Binding Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/IRF5 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/IRF7 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Interferon Regulatory Factor-7,
http://linkedlifedata.com/resource/pubmed/chemical/Interferon Regulatory Factors,
http://linkedlifedata.com/resource/pubmed/chemical/Interferon-beta,
http://linkedlifedata.com/resource/pubmed/chemical/Interferons,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Complementary,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger,
http://linkedlifedata.com/resource/pubmed/chemical/Transcription Factors
|
|
pubmed:status |
MEDLINE
|
|
pubmed:month |
Oct
|
|
pubmed:issn |
0021-9258
|
|
pubmed:author |
|
|
pubmed:issnType |
Print
|
|
pubmed:day |
22
|
|
pubmed:volume |
279
|
|
pubmed:owner |
NLM
|
|
pubmed:authorsComplete |
Y
|
|
pubmed:pagination |
45194-207
|
|
pubmed:dateRevised |
2007-11-15
|
|
pubmed:meshHeading |
pubmed-meshheading:15308637-Blotting, Western,
pubmed-meshheading:15308637-Carbon Tetrachloride,
pubmed-meshheading:15308637-Cell Line,
pubmed-meshheading:15308637-Cell Line, Tumor,
pubmed-meshheading:15308637-Chemokine CXCL11,
pubmed-meshheading:15308637-Chemokines, CXC,
pubmed-meshheading:15308637-DNA,
pubmed-meshheading:15308637-DNA, Complementary,
pubmed-meshheading:15308637-DNA-Binding Proteins,
pubmed-meshheading:15308637-Dendritic Cells,
pubmed-meshheading:15308637-Electrophoresis, Polyacrylamide Gel,
pubmed-meshheading:15308637-Gene Expression Regulation,
pubmed-meshheading:15308637-Humans,
pubmed-meshheading:15308637-Inflammation,
pubmed-meshheading:15308637-Interferon Regulatory Factor-7,
pubmed-meshheading:15308637-Interferon Regulatory Factors,
pubmed-meshheading:15308637-Interferon-beta,
pubmed-meshheading:15308637-Interferons,
pubmed-meshheading:15308637-Kinetics,
pubmed-meshheading:15308637-Mitochondria,
pubmed-meshheading:15308637-Monocytes,
pubmed-meshheading:15308637-Nucleic Acid Hybridization,
pubmed-meshheading:15308637-Oligonucleotide Array Sequence Analysis,
pubmed-meshheading:15308637-Phosphorylation,
pubmed-meshheading:15308637-RNA, Complementary,
pubmed-meshheading:15308637-RNA, Messenger,
pubmed-meshheading:15308637-Reverse Transcriptase Polymerase Chain Reaction,
pubmed-meshheading:15308637-Time Factors,
pubmed-meshheading:15308637-Transcription, Genetic,
pubmed-meshheading:15308637-Transcription Factors,
pubmed-meshheading:15308637-Up-Regulation
|
|
pubmed:year |
2004
|
|
pubmed:articleTitle |
Global and distinct targets of IRF-5 and IRF-7 during innate response to viral infection.
|
|
pubmed:affiliation |
The Sidney Kimmel Comprehensive Cancer Center, University of Michigan, Ann Arbor 48109, USA.
|
|
pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
|
