Linked Life Data

15306545

Source:http://linkedlifedata.com/resource/pubmed/id/15306545

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
6
pubmed:dateCreated
2004-11-4
pubmed:abstractText
We used the short-circuit current (I(sc)) technique to investigate the effects of the isoflavone genistein on the electrogenic Cl(-) secretion of the mouse jejunum. Genistein stimulated a sustained increase in I(sc) that was dose dependent. Bumetanide inhibited 76 +/- 5% of the genistein-stimulated I(sc) consistent with activation of Cl(-) secretion. Genistein failed to stimulate I(sc) following maximal activation of the cAMP pathway by forskolin. In addition, forskolin had a reduced effect on I(sc) of the mouse jejunum in the presence of genistein. Glibenclamide, a blocker of CFTR, eliminated the genistein-stimulated increase of I(sc) and reduced the forskolin-activated I(sc). Clotrimazole, a Ca(2+)-activated K(+) channel blocker, failed to reduce the genistein-stimulated I(sc). Vanadate, a blocker of tyrosine-dependent phosphatases, reduced the genistein-activated I(sc). Tyrphostin A23, a tyrosine kinase inhibitor, reduced basal I(sc), after which genistein failed to stimulate I(sc). These data suggest that genistein activated a sustained Cl(-) secretory response of the mouse jejunum and that the effect of genistein was via a tyrosine-dependent phosphorylation pathway.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Dec
pubmed:issn
0363-6143
pubmed:author
pubmed:issnType
Print
pubmed:volume
287
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
C1636-45
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
2004
pubmed:articleTitle
Genistein stimulates electrogenic Cl(-) secretion in mouse jejunum.
pubmed:affiliation
Department of Physiology, School of Medical Sciences, University of Otago, PO Box 913, Dunedin, New Zealand.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't