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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
2
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pubmed:dateCreated |
1992-10-22
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pubmed:abstractText |
Partially purified recombinant human Platelet-derived Growth Factor BB homodimer isolated from yeast culture media contains variable amounts of unprocessed PDGF-BB. This unprocessed PDGF-BB is found as a result of incomplete cleavage of the precursor to form the mature protein. Although the signal peptide is efficiently removed, a fraction of the PDGF secreted has an extended sequence corresponding to the truncated yeast alpha-factor leader. The data suggest that it is the amino acid chain from the truncated a-factor leader and not the sugar moiety attached to it that is responsible for the higher mitogenic activity found in this unprocessed molecule compared to highly purified PDGF-BB.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Sep
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pubmed:issn |
0006-291X
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
16
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pubmed:volume |
187
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1193-9
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pubmed:dateRevised |
2004-11-17
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pubmed:meshHeading |
pubmed-meshheading:1530614-Amino Acid Sequence,
pubmed-meshheading:1530614-Blotting, Western,
pubmed-meshheading:1530614-Glycosylation,
pubmed-meshheading:1530614-Humans,
pubmed-meshheading:1530614-Macromolecular Substances,
pubmed-meshheading:1530614-Mitogens,
pubmed-meshheading:1530614-Molecular Sequence Data,
pubmed-meshheading:1530614-Molecular Weight,
pubmed-meshheading:1530614-Platelet-Derived Growth Factor,
pubmed-meshheading:1530614-Protein Precursors,
pubmed-meshheading:1530614-Protein Sorting Signals,
pubmed-meshheading:1530614-Recombinant Proteins,
pubmed-meshheading:1530614-Saccharomyces cerevisiae,
pubmed-meshheading:1530614-Structure-Activity Relationship
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pubmed:year |
1992
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pubmed:articleTitle |
Incomplete process of recombinant human platelet-derived growth factor produced in yeast and its effect on the biological activity.
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pubmed:affiliation |
Chiron Research Laboratories, Chiron Corporation, Emeryville, California 94608.
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pubmed:publicationType |
Journal Article
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