Source:http://linkedlifedata.com/resource/pubmed/id/15305153
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
4
|
| pubmed:dateCreated |
2004-8-12
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| pubmed:abstractText |
As previous studies suggested the expression of a 12-LOX enzyme in murine and human melanoma cell lines, the primary aim of this project was to genetically identify the 12-LOX enzyme (platelet-, leukocyte- or epithelial form). By using reverse transcriptase-polymerase chain reaction, sequencing and various immunological techniques we have demonstrated conclusively the expression of the platelet-type 12-LOX in human melanoma cells of different origin, in their transplanted xenografts and in fresh human skin tumors. Furthermore, we found that p12-LOX is able to provide a survival signal for melanoma cells since inhibition of the enzyme by general LOX or selective 12-LOX inhibitors induced apoptosis in vitro. p12-LOX of human melanoma has been shown to be involved in the control of the metastatic phenotype, since we have detected the upregulation of the 12-LOX protein expression in spontaneously metastasizing xenografts and in thick human skin tumors (> 3.0 mm) characterized by high risk for the development of metastasis. Co-expression of two megakaryocytic genes, p12-LOX and alphaIIb integrin chains, was found to be a frequent phenomenon in human melanoma (approximately 70%) suggesting a common regulatory defect in this tumor.
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| pubmed:grant | |
| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Aug
|
| pubmed:issn |
0960-8931
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| pubmed:author | |
| pubmed:copyrightInfo |
Copyright 2004 Lippincott Williams & Wilkins
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| pubmed:issnType |
Print
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| pubmed:volume |
14
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| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
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| pubmed:pagination |
245-50
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| pubmed:dateRevised |
2007-11-14
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| pubmed:meshHeading |
pubmed-meshheading:15305153-Animals,
pubmed-meshheading:15305153-Apoptosis,
pubmed-meshheading:15305153-Arachidonate 12-Lipoxygenase,
pubmed-meshheading:15305153-Blood Platelets,
pubmed-meshheading:15305153-Cell Line, Tumor,
pubmed-meshheading:15305153-Disease Progression,
pubmed-meshheading:15305153-Enzyme Inhibitors,
pubmed-meshheading:15305153-Humans,
pubmed-meshheading:15305153-Immunohistochemistry,
pubmed-meshheading:15305153-Integrins,
pubmed-meshheading:15305153-Melanoma,
pubmed-meshheading:15305153-Mice,
pubmed-meshheading:15305153-Microscopy, Confocal,
pubmed-meshheading:15305153-Neoplasm Metastasis,
pubmed-meshheading:15305153-Neoplasm Transplantation,
pubmed-meshheading:15305153-Platelet Membrane Glycoprotein IIb,
pubmed-meshheading:15305153-Rats,
pubmed-meshheading:15305153-Skin Neoplasms,
pubmed-meshheading:15305153-Transplantation, Heterologous
|
| pubmed:year |
2004
|
| pubmed:articleTitle |
Molecular identification, localization and function of platelet-type 12-lipoxygenase in human melanoma progression, under experimental and clinical conditions.
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| pubmed:affiliation |
Department of Tumor Progression, National Institute of Oncology, Budapest, Hungary.
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| pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
|