Source:http://linkedlifedata.com/resource/pubmed/id/15304965
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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
2
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pubmed:dateCreated |
2004-8-12
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pubmed:abstractText |
Ionizing radiation generates diverse DNA lesions that differentially induce cell death and mutations. In the present study, calf thymus DNA (400 microg/ml) and HeLa cells were irradiated by (60)Co gamma-rays, and abasic (AP) sites and endonuclease (Endo)III- and 8-oxoguanine glycosylase (hOGG1)-sensitive base modifications in DNA were quantitated by the aldehyde reactive probe (ARP) assay. The irradiation of calf thymus DNA in phosphate buffer generated 91 Endo III- and 100 hOGG1-sensitive base modifications and 110 AP sites per 10(6) base pairs (bp) per Gy. The yield of the lesions in Tris buffer was 41- to 91-fold lower than that in phosphate, demonstrating a radioprotective effect of Tris. The HeLa cell chromosomal DNA contained 12 Endo III- and 3.8 hOGG1-sensitive base modifications and less than 1 AP sites per 10(6) bp as endogenous damage, and their level was increased by irradiation. The yields of the damage at 1 Gy (roughly equivalent to the lethal dose of HeLa cells [1.6-1.8 Gy]) were 0.13 Endo III, 0.091 hOGG1, and 0.065 AP sites per 10(6) bp, showing that irradiation with a lethal dose brought about only a marginal increase in base damage relative to an endogenous one. A comparison of the present data with those reported for DNA strand breaks supports the primary importance of double-strand breaks and clustered lesions as lethal damages formed by ionizing radiation.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Biotin,
http://linkedlifedata.com/resource/pubmed/chemical/DNA,
http://linkedlifedata.com/resource/pubmed/chemical/DNA-Formamidopyrimidine Glycosylase,
http://linkedlifedata.com/resource/pubmed/chemical/DNA-formamidopyrimidine...,
http://linkedlifedata.com/resource/pubmed/chemical/Deoxyribonuclease (Pyrimidine Dimer),
http://linkedlifedata.com/resource/pubmed/chemical/Escherichia coli Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/NTH protein, E coli,
http://linkedlifedata.com/resource/pubmed/chemical/O-(biotinylcarbazoylmethyl)hydroxyla...,
http://linkedlifedata.com/resource/pubmed/chemical/calf thymus DNA
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pubmed:status |
MEDLINE
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pubmed:month |
Jun
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pubmed:issn |
0449-3060
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
45
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
229-37
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:15304965-Base Pair Mismatch,
pubmed-meshheading:15304965-Biotin,
pubmed-meshheading:15304965-DNA,
pubmed-meshheading:15304965-DNA Damage,
pubmed-meshheading:15304965-DNA Mutational Analysis,
pubmed-meshheading:15304965-DNA-Formamidopyrimidine Glycosylase,
pubmed-meshheading:15304965-Deoxyribonuclease (Pyrimidine Dimer),
pubmed-meshheading:15304965-Dose-Response Relationship, Radiation,
pubmed-meshheading:15304965-Escherichia coli Proteins,
pubmed-meshheading:15304965-HeLa Cells,
pubmed-meshheading:15304965-Humans,
pubmed-meshheading:15304965-Radiation Dosage
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pubmed:year |
2004
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pubmed:articleTitle |
Detection of endonuclease III- and 8-oxoguanine glycosylase-sensitive base modifications in gamma-irradiated DNA and cells by the aldehyde reactive probe (ARP) assay.
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pubmed:affiliation |
Department of Mathematical and Life Sciences, Graduate School of Science, Hiroshima University, Higashi-Hiroshima, Japan.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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