Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
33
pubmed:dateCreated
2004-8-18
pubmed:abstractText
Transmissible spongiform encephalopathies arise as a consequence of infection of the central nervous system by prions, where neurons and glial cells are regarded as primary targets. Neuronal loss and gliosis, associated with the accumulation of misfolded prion protein (PrP), are hallmarks of prion diseases; yet the mechanisms underlying such disorders remain unclear. Here we introduced a cell system based on primary cerebellar cultures established from transgenic mice expressing ovine PrP and then exposed to sheep scrapie agent. Upon exposure to low doses of infectious agent, such cultures, unlike cultures originating from PrP null mice, were found to accumulate de novo abnormal PrP and infectivity, as assessed by mouse bioassay. Importantly, using astrocyte and neuron/astrocyte cocultures, both cell types were found capable of sustaining efficient prion propagation independently, leading to the production of proteinase K-resistant PrP of the same electrophoretic profile as in diseased brain. Moreover, contrasting with data obtained in chronically infected cell lines, late-occurring apoptosis was consistently demonstrated in the infected neuronal cultures. Our results provide evidence that primary cultured neural cells, including postmitotic neurons, are permissive to prion replication, thus establishing an approach to study the mechanisms involved in prion-triggered neurodegeneration at a cellular level.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/15302929-10590120, http://linkedlifedata.com/resource/pubmed/commentcorrection/15302929-11095725, http://linkedlifedata.com/resource/pubmed/commentcorrection/15302929-11259656, http://linkedlifedata.com/resource/pubmed/commentcorrection/15302929-11283320, http://linkedlifedata.com/resource/pubmed/commentcorrection/15302929-11309629, http://linkedlifedata.com/resource/pubmed/commentcorrection/15302929-11390599, http://linkedlifedata.com/resource/pubmed/commentcorrection/15302929-11417136, http://linkedlifedata.com/resource/pubmed/commentcorrection/15302929-11435592, http://linkedlifedata.com/resource/pubmed/commentcorrection/15302929-11488948, http://linkedlifedata.com/resource/pubmed/commentcorrection/15302929-11836421, http://linkedlifedata.com/resource/pubmed/commentcorrection/15302929-11937020, http://linkedlifedata.com/resource/pubmed/commentcorrection/15302929-12368333, http://linkedlifedata.com/resource/pubmed/commentcorrection/15302929-12655107, http://linkedlifedata.com/resource/pubmed/commentcorrection/15302929-12692301, http://linkedlifedata.com/resource/pubmed/commentcorrection/15302929-12821516, http://linkedlifedata.com/resource/pubmed/commentcorrection/15302929-1373228, http://linkedlifedata.com/resource/pubmed/commentcorrection/15302929-14522854, http://linkedlifedata.com/resource/pubmed/commentcorrection/15302929-14532116, http://linkedlifedata.com/resource/pubmed/commentcorrection/15302929-14581559, http://linkedlifedata.com/resource/pubmed/commentcorrection/15302929-14593181, http://linkedlifedata.com/resource/pubmed/commentcorrection/15302929-14671128, http://linkedlifedata.com/resource/pubmed/commentcorrection/15302929-14736847, http://linkedlifedata.com/resource/pubmed/commentcorrection/15302929-1671170, http://linkedlifedata.com/resource/pubmed/commentcorrection/15302929-1682507, http://linkedlifedata.com/resource/pubmed/commentcorrection/15302929-1693623, http://linkedlifedata.com/resource/pubmed/commentcorrection/15302929-3282080, http://linkedlifedata.com/resource/pubmed/commentcorrection/15302929-3528411, http://linkedlifedata.com/resource/pubmed/commentcorrection/15302929-6439820, http://linkedlifedata.com/resource/pubmed/commentcorrection/15302929-6808890, http://linkedlifedata.com/resource/pubmed/commentcorrection/15302929-7576660, http://linkedlifedata.com/resource/pubmed/commentcorrection/15302929-7642585, http://linkedlifedata.com/resource/pubmed/commentcorrection/15302929-7865744, http://linkedlifedata.com/resource/pubmed/commentcorrection/15302929-7901042, http://linkedlifedata.com/resource/pubmed/commentcorrection/15302929-8100741, http://linkedlifedata.com/resource/pubmed/commentcorrection/15302929-8464494, http://linkedlifedata.com/resource/pubmed/commentcorrection/15302929-8552188, http://linkedlifedata.com/resource/pubmed/commentcorrection/15302929-8598929, http://linkedlifedata.com/resource/pubmed/commentcorrection/15302929-8635458, http://linkedlifedata.com/resource/pubmed/commentcorrection/15302929-9032664, http://linkedlifedata.com/resource/pubmed/commentcorrection/15302929-9321385, http://linkedlifedata.com/resource/pubmed/commentcorrection/15302929-9343242, http://linkedlifedata.com/resource/pubmed/commentcorrection/15302929-9669696, http://linkedlifedata.com/resource/pubmed/commentcorrection/15302929-9811807, http://linkedlifedata.com/resource/pubmed/commentcorrection/15302929-9853120, http://linkedlifedata.com/resource/pubmed/commentcorrection/15302929-9867802
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Aug
pubmed:issn
0027-8424
pubmed:author
pubmed:issnType
Print
pubmed:day
17
pubmed:volume
101
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
12271-6
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
2004
pubmed:articleTitle
Prions can infect primary cultured neurons and astrocytes and promote neuronal cell death.
pubmed:affiliation
Unité de Virologie Immunologie Moléculaires, Institut National de la Recherche Agronomique, 78350 Jouy-en-Josas, France.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't