Switch to
| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
3
|
| pubmed:dateCreated |
1992-10-22
|
| pubmed:abstractText |
The effects of zinc deficiency were studied in mice submandibular salivary glands (SMG). Zn-restricted mice (Zn-) were maintained from weaning until adult age (60 days) with a powdered diet containing 3 mg Zn2+/kg. Pair-fed animals (30 mg Zn2+/kg powdered diet) and control animals fed a regular pelleted diet were also used. Total protein content and proteolytic activity of SMG did not differ among the groups, but morphometric evaluations revealed significant alterations in the nucleus/cytoplasm size ratios, most likely due to an absolute reduction in nuclear volume (control = 122.5 +/- 6.4; Zn- = 91.6 +/- 10.5; pair-fed = 125.1 +/- 6.8 microns 3) paralleled by an increase of the height of the duct epithelium (control = 70.5 +/- 3.0; Zn- = 90.5 +/- 4.2; pair-fed = 81.7 +/- 3.0 microns). The altered food consistency could be responsible for these morphological changes. In order to assess the subcellular distribution of SMG androgen receptors in conditions of chronic Zn deficiency, Zn- animals were mated and the F1 generation was fed as their dams until the age of 45 days. Cytosolic (in 105,000 g supernatants) and nuclear (KCl-extracted) SMG receptors were determined with [3H]R1881. The Zn- animals had reduced nuclear/cytosolic ratios of androgen receptors (control = 0.62; Zn- = 0.14), as an indication that chronically deficient Zn intake determines a sort of destabilization of the interactions of androgen-receptor complexes with target cell nucleus.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:issn |
0250-6807
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
36
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
167-74
|
| pubmed:dateRevised |
2006-11-15
|
| pubmed:meshHeading |
pubmed-meshheading:1530286-Androgens,
pubmed-meshheading:1530286-Animals,
pubmed-meshheading:1530286-Cell Nucleus,
pubmed-meshheading:1530286-Cytoplasm,
pubmed-meshheading:1530286-Feces,
pubmed-meshheading:1530286-Female,
pubmed-meshheading:1530286-Male,
pubmed-meshheading:1530286-Mice,
pubmed-meshheading:1530286-Organ Size,
pubmed-meshheading:1530286-Receptors, Androgen,
pubmed-meshheading:1530286-Submandibular Gland,
pubmed-meshheading:1530286-Testosterone,
pubmed-meshheading:1530286-Zinc
|
| pubmed:year |
1992
|
| pubmed:articleTitle |
Androgenic expression in the submandibular gland of zinc-deficient mice.
|
| pubmed:affiliation |
Department of Histology/Embryology, Universidade de São Paulo, Brazil.
|
| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|