Linked Life Data

15302402

Source:http://linkedlifedata.com/resource/pubmed/id/15302402

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
2004-8-10
pubmed:abstractText
The G-protein-coupled receptors (GPCRs) are a large superfamily of seven transmembrane domain-spanning proteins that mediate signal transduction by activation of G-proteins. Mammalian GPCR genes are reputed to be largely intron-deficient, but there have been no reports of using genome-wide analyses of gene structure to investigate this. Using complete genome sequences, we analysed the intron content of over 850 members of the rhodopsin-like GPCR subfamily (family A G-protein-coupled receptor or GPCR-A) in four species. We find that mouse and human GPCR-As have a large and significant reduction in intron number compared to the rest of their genome. In contrast, invertebrate GPCR-As have an intron repertoire similar to, or slightly greater than, the rest of their genome, suggesting that the reduced intron content in mammals is due to widespread intron loss. Furthermore, we provide a specific example of intron loss through analysis of an intron that is conserved in position and phase within a phylogenetically diverse range of GPCR-As within six vertebrate and invertebrate species. Together, these two lines of evidence provide compelling evidence for the widespread loss of introns during the evolution of the mammalian GPCR-A family.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Aug
pubmed:issn
0378-1119
pubmed:author
pubmed:issnType
Print
pubmed:day
18
pubmed:volume
338
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
15-23
pubmed:dateRevised
2005-11-17
pubmed:meshHeading
pubmed:year
2004
pubmed:articleTitle
Large-scale analysis of gene structure in rhodopsin-like GPCRs: evidence for widespread loss of an ancient intron.
pubmed:affiliation
MRC Human Genetics Unit, Western General Hospital, Edinburgh, EH4 2XU, UK.
pubmed:publicationType
Journal Article