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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
1-2
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pubmed:dateCreated |
2004-8-10
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pubmed:abstractText |
The aim of this study was to determine the effects of mouse granulocyte-macrophage colony-stimulating factor (mGM-CSF) on development of porcine parthenotes and nuclear transferred embryos, and on their expression of implantation-related genes. In the presence of bovine serum albumin, mGM-CSF did not increase the percentage of oocytes that developed to the blastocyst stage and at day 7 did not increase cell numbers of embryos. Addition of 2 ng/ml GM-CSF to protein-free culture medium significantly increased the compaction and blastocoel formation of 1- to 2-cell parthenotes developing in vitro. However, total cell numbers were not increased when they were cultured in the presence of GM-CSF. Semi-quantitative reverse transcriptase polymerase chain reaction revealed that mGM-CSF enhances mRNA expression of the leukemia inhibitory factor receptor, but does not influence interleukin-6 or sodium/glucose co-transporter protein gene expression in blastocyst stage parthenotes. These results suggest that mGM-CSF may enhance viability of porcine embryos developing in vitro in a defined culture medium.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Granulocyte-Macrophage...,
http://linkedlifedata.com/resource/pubmed/chemical/Interleukin-6,
http://linkedlifedata.com/resource/pubmed/chemical/Leukemia Inhibitory Factor...,
http://linkedlifedata.com/resource/pubmed/chemical/Lifr protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/Membrane Glycoproteins,
http://linkedlifedata.com/resource/pubmed/chemical/Monosaccharide Transport Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Cytokine,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, OSM-LIF,
http://linkedlifedata.com/resource/pubmed/chemical/Serum Albumin, Bovine,
http://linkedlifedata.com/resource/pubmed/chemical/Sodium-Glucose Transporter 1
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pubmed:status |
MEDLINE
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pubmed:month |
Aug
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pubmed:issn |
0378-4320
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
84
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
169-77
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pubmed:dateRevised |
2007-11-15
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pubmed:meshHeading |
pubmed-meshheading:15302396-Animals,
pubmed-meshheading:15302396-Blastocyst,
pubmed-meshheading:15302396-Culture Techniques,
pubmed-meshheading:15302396-Electric Stimulation,
pubmed-meshheading:15302396-Embryo, Mammalian,
pubmed-meshheading:15302396-Embryo Implantation,
pubmed-meshheading:15302396-Embryonic Development,
pubmed-meshheading:15302396-Gene Expression,
pubmed-meshheading:15302396-Granulocyte-Macrophage Colony-Stimulating Factor,
pubmed-meshheading:15302396-Interleukin-6,
pubmed-meshheading:15302396-Leukemia Inhibitory Factor Receptor alpha Subunit,
pubmed-meshheading:15302396-Membrane Glycoproteins,
pubmed-meshheading:15302396-Mice,
pubmed-meshheading:15302396-Monosaccharide Transport Proteins,
pubmed-meshheading:15302396-Oocytes,
pubmed-meshheading:15302396-Parthenogenesis,
pubmed-meshheading:15302396-RNA, Messenger,
pubmed-meshheading:15302396-Receptors, Cytokine,
pubmed-meshheading:15302396-Receptors, OSM-LIF,
pubmed-meshheading:15302396-Reverse Transcriptase Polymerase Chain Reaction,
pubmed-meshheading:15302396-Serum Albumin, Bovine,
pubmed-meshheading:15302396-Sodium-Glucose Transporter 1,
pubmed-meshheading:15302396-Swine
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pubmed:year |
2004
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pubmed:articleTitle |
Mouse granulocyte-macrophage colony-stimulating factor enhances viability of porcine embryos in defined culture conditions.
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pubmed:affiliation |
Department of Animal Sciences, Chungbuk National University, Gaesin-dong, Cheongju, Chungbuk 361-763, South Korea.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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