Linked Life Data

15297311

Source:http://linkedlifedata.com/resource/pubmed/id/15297311

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
10
pubmed:dateCreated
2004-11-4
pubmed:abstractText
Transcription factor GATA-1 is required for erythropoiesis, yet its full actions are unknown. We performed transcriptome analysis of G1E-ER4 cells, a GATA-1-null erythroblast line that undergoes synchronous erythroid maturation when GATA-1 activity is restored. We interrogated more than 9000 transcripts at 6 time points representing the transition from late burst forming unit-erythroid (BFU-E) to basophilic erythroblast stages. Our findings illuminate several new aspects of GATA-1 function. First, the large number of genes responding quickly to restoration of GATA-1 extends the repertoire of its potential targets. Second, many transcripts were rapidly down-regulated, highlighting the importance of GATA-1 in gene repression. Third, up-regulation of some known GATA-1 targets was delayed, suggesting that auxiliary factors are required. For example, induction of the direct GATA-1 target gene beta major globin was late and, surprisingly, required new protein synthesis. In contrast, the gene encoding Fog1, which cooperates with GATA-1 in beta globin transcription, was rapidly induced independently of protein synthesis. Guided by bioinformatic analysis, we demonstrated that selected regions of the Fog1 gene exhibit enhancer activity and in vivo occupancy by GATA-1. These findings define a regulatory loop for beta globin expression and, more generally, demonstrate how transcriptome analysis can be used to generate testable hypotheses regarding transcriptional networks.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
AIM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Nov
pubmed:issn
0006-4971
pubmed:author
pubmed:issnType
Print
pubmed:day
15
pubmed:volume
104
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
3136-47
pubmed:dateRevised
2008-11-21
pubmed:meshHeading
pubmed-meshheading:15297311-Animals, pubmed-meshheading:15297311-Carrier Proteins, pubmed-meshheading:15297311-Cell Line, pubmed-meshheading:15297311-DNA-Binding Proteins, pubmed-meshheading:15297311-Enhancer Elements, Genetic, pubmed-meshheading:15297311-Erythroblasts, pubmed-meshheading:15297311-Erythroid-Specific DNA-Binding Factors, pubmed-meshheading:15297311-Erythropoiesis, pubmed-meshheading:15297311-GATA1 Transcription Factor, pubmed-meshheading:15297311-Gene Expression Profiling, pubmed-meshheading:15297311-Genetic Complementation Test, pubmed-meshheading:15297311-Humans, pubmed-meshheading:15297311-Mice, pubmed-meshheading:15297311-Nuclear Proteins, pubmed-meshheading:15297311-Reproducibility of Results, pubmed-meshheading:15297311-Transcription, Genetic, pubmed-meshheading:15297311-Transcription Factors
pubmed:year
2004
pubmed:articleTitle
Global regulation of erythroid gene expression by transcription factor GATA-1.
pubmed:affiliation
The Children's Hospital of Philadelphia, Division of Hematology, University of Pennsylvania, Philadelphia, PA 19104, USA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't