Source:http://linkedlifedata.com/resource/pubmed/id/15296850
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
3
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| pubmed:dateCreated |
2004-8-6
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| pubmed:abstractText |
In ischemic preconditioning (IPC), brief sublethal ischemia protects neurons from a subsequent lethal ischemia. In vivo models faithfully display preconditioning, yet, these models are technically challenging, time-consuming and expensive. In vitro models of preconditioning have also been developed that are technically easier and less expensive. A drawback of pre-existing in vitro models is that since susceptibility to ischemic injury is age-dependent; neuroprotection is being studied in neurons that have intrinsic resistance to oxygen-glucose deprivation (OGD). This study introduces a new in vitro model of ischemic preconditioning in hippocampal slice cultures isolated from 20-30-day-old rats. Slice cultures show a high susceptibility and sharp thresholds toward ischemia that is comparable to that found in vivo. A 5-min OGD treatment was not neurotoxic to young adult slice cultures, while a 10-min OGD treatment was neurotoxic. In addition, the sublethal 5-min OGD treatment protected against a 10-min OGD treatment that was delivered 24 h later. Neuroprotection was seen in preconditioned slice cultures stained with propidium iodide (PI) or with antisera against the neuron-specific antigen NeuN. Energy failure is hypothesized to trigger ischemic preconditioning and a 5-min OGD treatment induced transient energy failure in young adult slice cultures. This model may assist in the search for new therapeutics for the prevention and/or treatment of stroke.
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| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Adenosine Triphosphate,
http://linkedlifedata.com/resource/pubmed/chemical/Antibodies,
http://linkedlifedata.com/resource/pubmed/chemical/Glucose,
http://linkedlifedata.com/resource/pubmed/chemical/Nerve Tissue Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/NeuN protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/Neuroprotective Agents,
http://linkedlifedata.com/resource/pubmed/chemical/Nuclear Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Propidium
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| pubmed:status |
MEDLINE
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| pubmed:month |
Aug
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| pubmed:issn |
1385-299X
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| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
13
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| pubmed:owner |
NLM
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| pubmed:authorsComplete |
Y
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| pubmed:pagination |
135-43
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| pubmed:dateRevised |
2006-11-15
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| pubmed:meshHeading |
pubmed-meshheading:15296850-Adenosine Triphosphate,
pubmed-meshheading:15296850-Animals,
pubmed-meshheading:15296850-Antibodies,
pubmed-meshheading:15296850-Brain Ischemia,
pubmed-meshheading:15296850-Diffusion Chambers, Culture,
pubmed-meshheading:15296850-Energy Metabolism,
pubmed-meshheading:15296850-Glucose,
pubmed-meshheading:15296850-Hippocampus,
pubmed-meshheading:15296850-Hypoxia, Brain,
pubmed-meshheading:15296850-Ischemic Preconditioning,
pubmed-meshheading:15296850-Models, Biological,
pubmed-meshheading:15296850-Nerve Tissue Proteins,
pubmed-meshheading:15296850-Neuroprotective Agents,
pubmed-meshheading:15296850-Nuclear Proteins,
pubmed-meshheading:15296850-Organ Culture Techniques,
pubmed-meshheading:15296850-Propidium,
pubmed-meshheading:15296850-Rats,
pubmed-meshheading:15296850-Rats, Sprague-Dawley
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| pubmed:year |
2004
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| pubmed:articleTitle |
A new model of ischemic preconditioning using young adult hippocampal slice cultures.
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| pubmed:affiliation |
Department of Physiology and Pharmacology, State University New York-Downstate Medical Center, 450 Clarkson Avenue, Brooklyn, NY 11203, USA.
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| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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