Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
15
pubmed:dateCreated
2004-8-6
pubmed:databankReference
pubmed:abstractText
In migrating cells, the actin filament nucleation activity of ARP2/3 is an essential component of dynamic cell shape change and motility. In response to signals from the small GTPase Rac1, alterations in the composition and/or subcellular localization of the WAVE complex lead to ARP2/3 activation. The human WAVE complex subunit, WAVE1/SCAR1, was first identified in Dictyostelium and is a direct ARP2/3 activator. In the absence of an intact WAVE complex, SCAR/WAVE protein is destabilized. Although the composition of the five-subunit WAVE complex is well characterized, the means by which individual subunits and fully assembled WAVE complexes regulate ARP2/3 in vivo are unclear. The molecular genetics of trichome distortion in Arabidopsis is a powerful system to understand how signaling pathways and ARP2/3 control multicellular development. In this paper we prove that the GNARLED gene encodes a homolog of the WAVE subunit NAP125. Despite the moderate level of amino acid identity between Arabidopsis and human NAP125, both homologs were functionally interchangeable in vivo and interacted physically with the putative Arabidopsis WAVE subunit ATSRA1. gnarled trichomes had nearly identical cell shape and actin cytoskeleton phenotypes when compared to ARP2/3 subunit mutants, suggesting that GRL positively regulates ARP2/3.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Aug
pubmed:issn
0960-9822
pubmed:author
pubmed:issnType
Print
pubmed:day
10
pubmed:volume
14
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1405-9
pubmed:dateRevised
2006-11-20
pubmed:meshHeading
pubmed-meshheading:15296760-Actin-Related Protein 2, pubmed-meshheading:15296760-Actin-Related Protein 2-3 Complex, pubmed-meshheading:15296760-Actin-Related Protein 3, pubmed-meshheading:15296760-Actins, pubmed-meshheading:15296760-Alleles, pubmed-meshheading:15296760-Amino Acid Sequence, pubmed-meshheading:15296760-Arabidopsis, pubmed-meshheading:15296760-Arabidopsis Proteins, pubmed-meshheading:15296760-Base Sequence, pubmed-meshheading:15296760-Blotting, Western, pubmed-meshheading:15296760-Cytoskeleton, pubmed-meshheading:15296760-DNA Primers, pubmed-meshheading:15296760-Microfilament Proteins, pubmed-meshheading:15296760-Microscopy, Electron, Scanning, pubmed-meshheading:15296760-Molecular Sequence Data, pubmed-meshheading:15296760-Mutation, pubmed-meshheading:15296760-Plant Leaves, pubmed-meshheading:15296760-Plasmids, pubmed-meshheading:15296760-Reverse Transcriptase Polymerase Chain Reaction, pubmed-meshheading:15296760-Sequence Alignment, pubmed-meshheading:15296760-Sequence Analysis, DNA, pubmed-meshheading:15296760-Two-Hybrid System Techniques, pubmed-meshheading:15296760-Wiskott-Aldrich Syndrome Protein Family
pubmed:year
2004
pubmed:articleTitle
Arabidopsis GNARLED encodes a NAP125 homolog that positively regulates ARP2/3.
pubmed:affiliation
Agronomy Department, 915 W. State Street, West Lafayette, IN 47907, USA.
pubmed:publicationType
Journal Article, Comparative Study, Research Support, U.S. Gov't, Non-P.H.S., Research Support, Non-U.S. Gov't