Linked Life Data

15295877

Source:http://linkedlifedata.com/resource/pubmed/id/15295877

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
2004-8-5
pubmed:abstractText
Rabeprazole sodium is a proton pump inhibitor that covalently binds and inactivates the gastric parietal cell proton pump (H+/K+ ATPase). Little has been published about the quantitative determination of this drug. The aim of this research was to develop a new liquid chromatographic method for quantitative determination of rabeprazole in coated tablets. The system consisted of a Hypersil Keystone Betabasic C8 column (250 x 4.6 mm, 5 microm particle size), an isocratic acetonitrile-water (35 + 65) mobile phase at a flow rate of 1.0 mL/min, and a diode array detector set at 282 nm. The following validation parameters were evaluated: linearity, precision, accuracy, specificity, detection and quantitation limits, and robustness. The method showed good linearity in the concentration range of 10-70 microg/mL. The quantitation limit was 2.43 microg/mL, and the detection limit was 0.80 microg/mL. The intra- and interday precision data showed that the method has good reproducibility (relative standard deviation = 1.03). Accuracy and robustness were also evaluated, and the results were satisfactory. The mean recovery was 101.61%. The analysis of a placebo mixture demonstrated the method is also specific.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
1060-3271
pubmed:author
pubmed:issnType
Print
pubmed:volume
87
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
842-6
pubmed:dateRevised
2008-3-17
pubmed:meshHeading
pubmed:articleTitle
New liquid chromatographic method for determination of rabeprazole sodium in coated tablets.
pubmed:affiliation
Universidade Federal do Rio Grande do Sul., Programa de Pós-graduação em Ciências Farmacêuticas, Faculdade de Farmácia, Av. Ipiranga, Porto Alegre/RS CEP, Brazil. cassiavgarcia@yahoo.com.br
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't