Linked Life Data

15294897

Source:http://linkedlifedata.com/resource/pubmed/id/15294897

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
42
pubmed:dateCreated
2004-10-11
pubmed:abstractText
Two cis-acting RNA trafficking sequences (heterogenous ribonucleoprotein A2 (hnRNP A2)-response elements 1 and 2 or A2RE-1 and A2RE-2) have been identified in HIV-1 vpr and gag mRNAs and were found to confer cytoplasmic RNA trafficking in a murine oligodendrocyte assay. Their activities were assessed during HIV-1 proviral gene expression in COS7 cells. Single point mutations that were shown to severely block RNA trafficking were introduced into each of the A2REs. In both cases, this resulted in a marked decrease in hnRNP A2 binding to HIV-1 genomic RNA in whole cell extracts and hnRNP A2-containing polysomes. This also resulted in an accumulation of HIV-1 genomic RNA in the nucleus and a significant reduction in genomic RNA encapsidation levels. Immunofluorescence analyses revealed altered expression patterns for pr55Gag and particularly that for Vpr. Vpr localization became almost completely nuclear and this was reflected in a significant reduction in virion-associated Vpr levels. These effects coincided with late steps of the viral replication cycle and were not seen at early time points post-transfection. Transcription, splicing, steady state RNA levels, and pr55Gag processing were not affected. On the other hand, viral replication was markedly compromised in A2RE-2 mutant viruses and this correlated with lowered genomic RNA encapsidation levels. These data reveal new insights into the virus-host interactions between hnRNP A2 and the HIV-1 A2REs and their influence on the patterns of HIV-1 gene expression and viral assembly.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Oct
pubmed:issn
0021-9258
pubmed:author
pubmed:issnType
Print
pubmed:day
15
pubmed:volume
279
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
44141-53
pubmed:dateRevised
2007-11-15
pubmed:meshHeading
pubmed-meshheading:15294897-Animals, pubmed-meshheading:15294897-Base Sequence, pubmed-meshheading:15294897-COS Cells, pubmed-meshheading:15294897-Cell Line, pubmed-meshheading:15294897-Cell Nucleus, pubmed-meshheading:15294897-Cercopithecus aethiops, pubmed-meshheading:15294897-DNA Primers, pubmed-meshheading:15294897-Gene Expression Regulation, Viral, pubmed-meshheading:15294897-Gene Products, gag, pubmed-meshheading:15294897-Gene Products, vpr, pubmed-meshheading:15294897-Genes, Viral, pubmed-meshheading:15294897-HIV-1, pubmed-meshheading:15294897-Heterogeneous-Nuclear Ribonucleoprotein Group A-B, pubmed-meshheading:15294897-Humans, pubmed-meshheading:15294897-In Situ Hybridization, Fluorescence, pubmed-meshheading:15294897-Proviruses, pubmed-meshheading:15294897-RNA, Messenger, pubmed-meshheading:15294897-RNA, Viral, pubmed-meshheading:15294897-Reverse Transcriptase Polymerase Chain Reaction, pubmed-meshheading:15294897-vpr Gene Products, Human Immunodeficiency Virus
pubmed:year
2004
pubmed:articleTitle
A late role for the association of hnRNP A2 with the HIV-1 hnRNP A2 response elements in genomic RNA, Gag, and Vpr localization.
pubmed:affiliation
HIV-1 RNA Trafficking Laboratory, Lady Davis Institute for Medical Research-Sir Mortimer B. Davis Jewish General Hospital, Room 323A, 3755 Côte-Ste-Catherine Road, Montréal, Québec H3T 1E2, Canada.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't