Linked Life Data

15294780

Source:http://linkedlifedata.com/resource/pubmed/id/15294780

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
8
pubmed:dateCreated
2004-8-5
pubmed:abstractText
We investigated the effects of copper on the structure and physiology of freshwater biofilm microbial communities. For this purpose, biofilms that were grown during 4 weeks in a shallow, slightly polluted ditch were exposed, in aquaria in our laboratory, to a range of copper concentrations (0, 1, 3, and 10 microM). Denaturing gradient gel electrophoresis (DGGE) revealed changes in the bacterial community in all aquaria. The extent of change was related to the concentration of copper applied, indicating that copper directly or indirectly caused the effects. Concomitantly with these changes in structure, changes in the metabolic potential of the heterotrophic bacterial community were apparent from changes in substrate use profiles as assessed on Biolog plates. The structure of the phototrophic community also changed during the experiment, as observed by microscopic analysis in combination with DGGE analysis of eukaryotic microorganisms and cyanobacteria. However, the extent of community change, as observed by DGGE, was not significantly greater in the copper treatments than in the control. Yet microscopic analysis showed a development toward a greater proportion of cyanobacteria in the treatments with the highest copper concentrations. Furthermore, copper did affect the physiology of the phototrophic community, as evidenced by the fact that a decrease in photosynthetic capacity was detected in the treatment with the highest copper concentration. Therefore, we conclude that copper affected the physiology of the biofilm and had an effect on the structure of the communities composing this biofilm.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/15294780-10347030, http://linkedlifedata.com/resource/pubmed/commentcorrection/15294780-11018124, http://linkedlifedata.com/resource/pubmed/commentcorrection/15294780-11257008, http://linkedlifedata.com/resource/pubmed/commentcorrection/15294780-11916688, http://linkedlifedata.com/resource/pubmed/commentcorrection/15294780-12206437, http://linkedlifedata.com/resource/pubmed/commentcorrection/15294780-12324315, http://linkedlifedata.com/resource/pubmed/commentcorrection/15294780-12434215, http://linkedlifedata.com/resource/pubmed/commentcorrection/15294780-12460271, http://linkedlifedata.com/resource/pubmed/commentcorrection/15294780-12545313, http://linkedlifedata.com/resource/pubmed/commentcorrection/15294780-12676715, http://linkedlifedata.com/resource/pubmed/commentcorrection/15294780-12703304, http://linkedlifedata.com/resource/pubmed/commentcorrection/15294780-14507367, http://linkedlifedata.com/resource/pubmed/commentcorrection/15294780-14602623, http://linkedlifedata.com/resource/pubmed/commentcorrection/15294780-7683183, http://linkedlifedata.com/resource/pubmed/commentcorrection/15294780-9097434, http://linkedlifedata.com/resource/pubmed/commentcorrection/15294780-9535661, http://linkedlifedata.com/resource/pubmed/commentcorrection/15294780-9925618
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Aug
pubmed:issn
0099-2240
pubmed:author
pubmed:issnType
Print
pubmed:volume
70
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
4512-21
pubmed:dateRevised
2010-11-18
pubmed:meshHeading
pubmed:year
2004
pubmed:articleTitle
Analysis of structural and physiological profiles to assess the effects of Cu on biofilm microbial communities.
pubmed:affiliation
Netherlands Institute of Ecology, Centre for Limnology, Rijkstraatweg 6, 3631 AC Nieuwersluis, The Netherlands. b.massieux@nioo.knaw.nl
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't