Linked Life Data

15293391

Source:http://linkedlifedata.com/resource/pubmed/id/15293391

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
7
pubmed:dateCreated
2004-8-5
pubmed:abstractText
Fluorescent-based single-strand conformation polymorphism (F-SSCP) analysis with capillary electrophoresis (CE) is the most common method for the detection of mutation because of its high sensitivity and resolution. In this study, we prepared an inexpensive linear polyacrylamide (LPA), and successfully applied it to CE-SSCP analysis and tandem CE-SSCP/heteroduplex analysis (HA) of the P53 gene on an ABI capillary genetic analyzer. A comparison of the sieving capabilities of a homemade LPA and commercial polydimethylacrylamide (PDMA) demonstrates that the homemade LPA has a higher resolution, a shorter analysis time, and is more suitable for tandem SSCP/HA than commercial PDMA. To show the usefulness, mutations of P53 gene exon 7 - 8 in 37 tumor samples were investigated by using homemade LPA. The results indicate that 10 mutations were found in 9 of 37 cases; the majority of P53 mutations were missense mutations, and 70% were located in exon 7, which plays an important role in neoplastic progression in human tumorigenesis.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jul
pubmed:issn
0910-6340
pubmed:author
pubmed:issnType
Print
pubmed:volume
20
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1001-5
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed-meshheading:15293391-Acrylic Resins, pubmed-meshheading:15293391-Codon, pubmed-meshheading:15293391-DNA Mutational Analysis, pubmed-meshheading:15293391-Exons, pubmed-meshheading:15293391-Fluorescent Dyes, pubmed-meshheading:15293391-Genes, p53, pubmed-meshheading:15293391-Humans, pubmed-meshheading:15293391-Mutation, pubmed-meshheading:15293391-Mutation, Missense, pubmed-meshheading:15293391-Nucleic Acid Heteroduplexes, pubmed-meshheading:15293391-Polymerase Chain Reaction, pubmed-meshheading:15293391-Polymers, pubmed-meshheading:15293391-Polymorphism, Single-Stranded Conformational, pubmed-meshheading:15293391-Sensitivity and Specificity, pubmed-meshheading:15293391-Sequence Analysis, DNA, pubmed-meshheading:15293391-Spectrometry, Fluorescence, pubmed-meshheading:15293391-Time Factors
pubmed:year
2004
pubmed:articleTitle
Fluorescent-based single-strand conformation polymorphism/heteroduplex capillary electrophoretic mutation analysis of the P53 gene.
pubmed:affiliation
National Chromatographic R&A Center, Dalian Institute of Chemical Physics, The Chinese Academy of Sciences, Dalian, China.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't