Linked Life Data

15292130

Source:http://linkedlifedata.com/resource/pubmed/id/15292130

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
16
pubmed:dateCreated
2004-8-4
pubmed:abstractText
Pseudomonas sp. VLB120 uses styrene as a sole source of carbon and energy. The first step in this metabolic pathway is catalyzed by an oxygenase (StyA) and a NADH-flavin oxidoreductase (StyB). Both components have been isolated from wild-type Pseudomonas strain VLB120 as well as from recombinant Escherichia coli. StyA from both sources is a dimer, with a subunit size of 47 kDa, and catalyzes the enantioselective epoxidation of CC double bonds. Styrene is exclusively converted to S-styrene oxide with a specific activity of 2.1 U mg(-1) (k(cat) = 1.6 s(-1)) and K(m) values for styrene of 0.45 +/- 0.05 mM (wild type) and 0.38 +/- 0.09 mM (recombinant). The epoxidation reaction depends on the presence of a NADH-flavin adenine dinucleotide (NADH-FAD) oxidoreductase for the supply of reduced FAD. StyB is a dimer with a molecular mass of 18 kDa and a NADH oxidation activity of 200 U mg(-1) (k(cat) [NADH] = 60 s(-1)). Steady-state kinetics determined for StyB indicate a mechanism of sequential binding of NADH and flavin to StyB. This enzyme reduces FAD as well as flavin mononucleotide and riboflavin. The NADH oxidation activity does not depend on the presence of StyA. During the epoxidation reaction, no formation of a complex of StyA and StyB has been observed, suggesting that electron transport between reductase and oxygenase occurs via a diffusing flavin.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/15292130-10347083, http://linkedlifedata.com/resource/pubmed/commentcorrection/15292130-10397854, http://linkedlifedata.com/resource/pubmed/commentcorrection/15292130-10480865, http://linkedlifedata.com/resource/pubmed/commentcorrection/15292130-10584030, http://linkedlifedata.com/resource/pubmed/commentcorrection/15292130-10633095, http://linkedlifedata.com/resource/pubmed/commentcorrection/15292130-10653707, http://linkedlifedata.com/resource/pubmed/commentcorrection/15292130-10666321, http://linkedlifedata.com/resource/pubmed/commentcorrection/15292130-10744688, http://linkedlifedata.com/resource/pubmed/commentcorrection/15292130-10820335, http://linkedlifedata.com/resource/pubmed/commentcorrection/15292130-11248689, http://linkedlifedata.com/resource/pubmed/commentcorrection/15292130-11283293, http://linkedlifedata.com/resource/pubmed/commentcorrection/15292130-11327836, http://linkedlifedata.com/resource/pubmed/commentcorrection/15292130-11683878, http://linkedlifedata.com/resource/pubmed/commentcorrection/15292130-11733527, http://linkedlifedata.com/resource/pubmed/commentcorrection/15292130-12115117, http://linkedlifedata.com/resource/pubmed/commentcorrection/15292130-12413667, http://linkedlifedata.com/resource/pubmed/commentcorrection/15292130-12436305, http://linkedlifedata.com/resource/pubmed/commentcorrection/15292130-12809507, http://linkedlifedata.com/resource/pubmed/commentcorrection/15292130-12837091, http://linkedlifedata.com/resource/pubmed/commentcorrection/15292130-12968028, http://linkedlifedata.com/resource/pubmed/commentcorrection/15292130-2037557, http://linkedlifedata.com/resource/pubmed/commentcorrection/15292130-2207083, http://linkedlifedata.com/resource/pubmed/commentcorrection/15292130-2272946, http://linkedlifedata.com/resource/pubmed/commentcorrection/15292130-2339888, http://linkedlifedata.com/resource/pubmed/commentcorrection/15292130-2682640, http://linkedlifedata.com/resource/pubmed/commentcorrection/15292130-3122838, http://linkedlifedata.com/resource/pubmed/commentcorrection/15292130-3417677, http://linkedlifedata.com/resource/pubmed/commentcorrection/15292130-4515004, http://linkedlifedata.com/resource/pubmed/commentcorrection/15292130-5438357, http://linkedlifedata.com/resource/pubmed/commentcorrection/15292130-7665508, http://linkedlifedata.com/resource/pubmed/commentcorrection/15292130-8017910, http://linkedlifedata.com/resource/pubmed/commentcorrection/15292130-8033996, http://linkedlifedata.com/resource/pubmed/commentcorrection/15292130-8077188, http://linkedlifedata.com/resource/pubmed/commentcorrection/15292130-8919815, http://linkedlifedata.com/resource/pubmed/commentcorrection/15292130-9287340, http://linkedlifedata.com/resource/pubmed/commentcorrection/15292130-9495743, http://linkedlifedata.com/resource/pubmed/commentcorrection/15292130-9603811, http://linkedlifedata.com/resource/pubmed/commentcorrection/15292130-9772191
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
http://linkedlifedata.com/resource/pubmed/chemical/Epoxy Compounds, http://linkedlifedata.com/resource/pubmed/chemical/FMN Reductase, http://linkedlifedata.com/resource/pubmed/chemical/Flavin Mononucleotide, http://linkedlifedata.com/resource/pubmed/chemical/Flavin-Adenine Dinucleotide, http://linkedlifedata.com/resource/pubmed/chemical/NAD, http://linkedlifedata.com/resource/pubmed/chemical/Oxygenases, http://linkedlifedata.com/resource/pubmed/chemical/Protein Subunits, http://linkedlifedata.com/resource/pubmed/chemical/Recombinant Proteins, http://linkedlifedata.com/resource/pubmed/chemical/Riboflavin, http://linkedlifedata.com/resource/pubmed/chemical/Styrene, http://linkedlifedata.com/resource/pubmed/chemical/styrene monooxygenase, http://linkedlifedata.com/resource/pubmed/chemical/styrene oxide
pubmed:status
MEDLINE
pubmed:month
Aug
pubmed:issn
0021-9193
pubmed:author
pubmed:issnType
Print
pubmed:volume
186
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
5292-302
pubmed:dateRevised
2010-9-21
pubmed:meshHeading
pubmed:year
2004
pubmed:articleTitle
Biochemical characterization of StyAB from Pseudomonas sp. strain VLB120 as a two-component flavin-diffusible monooxygenase.
pubmed:affiliation
Institute of Biotechnology, ETHZ, Swiss Federal Institute of Technology, ETH Hoenggerberg, HPT, CH-8093, Zurich, Switzerland.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't