Source:http://linkedlifedata.com/resource/pubmed/id/15289887
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
3
|
| pubmed:dateCreated |
2004-8-3
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| pubmed:abstractText |
This study describes two experimental models for the in vitro reconstitution of the human bladder mucosa (neo-bladder): human urothelial stabilized cell lines were cultured on three-dimensional matrices, collagen or platelet-fibrin gels, containing murine fibroblast 3T3-J2 cells. Low-density seeding (2x10(4) cells/ml) of both normal (TCA-48) and neoplastic cell lines (TCA-47) on collagen matrix gave rise to isolated papillar colonies, while high-density seeding (3.75x10(6) cells/ml) led to the formation of wide pluristratified epithelial sheets, resembling the normal transitional epithelium. In contrast, high-density seeding (5x10(5) cells/ml) on platelet-fibrin matrix did not allow the formation of epithelial sheets: only isolated voluminous colonies of normal TCA-48 cells, and sparse and small colonies of neoplastic TCA-47 could be observed. Growth assays and cytotoxicity reduction tests showed that the growth inhibitory effect of platelet-fibrin gel on urothelial cells was probably due to the aspecific activation of the complement contained in the plasmatic fraction, whose precipitation forms fibrin-glue. Collectively, these findings allow us to draw the following conclusions: i) neobladders obtained by culturing urothelial cells on collagen matrix reproduce normal bladder mucosa and could be utilized in pharmacological studies; and ii) platelet-fibrin gels, that specifically inhibit neoplastic urothelial cell growth, could be used as scaffolds in surgical bladder reconstitution.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Sep
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| pubmed:issn |
1107-3756
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| pubmed:author | |
| pubmed:issnType |
Print
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| pubmed:volume |
14
|
| pubmed:owner |
NLM
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| pubmed:authorsComplete |
Y
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| pubmed:pagination |
367-72
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| pubmed:dateRevised |
2006-11-15
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| pubmed:meshHeading |
pubmed-meshheading:15289887-3T3 Cells,
pubmed-meshheading:15289887-Animals,
pubmed-meshheading:15289887-Blood Platelets,
pubmed-meshheading:15289887-Cell Count,
pubmed-meshheading:15289887-Cell Line,
pubmed-meshheading:15289887-Cell Line, Tumor,
pubmed-meshheading:15289887-Cell Proliferation,
pubmed-meshheading:15289887-Cell Survival,
pubmed-meshheading:15289887-Collagen,
pubmed-meshheading:15289887-Culture Media,
pubmed-meshheading:15289887-Culture Techniques,
pubmed-meshheading:15289887-Extracellular Matrix,
pubmed-meshheading:15289887-Fibrin,
pubmed-meshheading:15289887-Fibroblasts,
pubmed-meshheading:15289887-Gels,
pubmed-meshheading:15289887-Humans,
pubmed-meshheading:15289887-Mice,
pubmed-meshheading:15289887-Mucous Membrane,
pubmed-meshheading:15289887-Time Factors,
pubmed-meshheading:15289887-Toxicity Tests,
pubmed-meshheading:15289887-Urinary Bladder,
pubmed-meshheading:15289887-Urinary Bladder Neoplasms,
pubmed-meshheading:15289887-Urothelium
|
| pubmed:year |
2004
|
| pubmed:articleTitle |
New experimental models for the in vitro reconstitution of human bladder mucosa.
|
| pubmed:affiliation |
Immunotransfusional Center, Cell Culture Laboratory, Treviso Regional Hospital, I-31100 Treviso, Italy.
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| pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, Non-U.S. Gov't
|