Linked Life Data

15287585

Source:http://linkedlifedata.com/resource/pubmed/id/15287585

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
2004-8-3
pubmed:abstractText
The Trypanosoma brucei reference strain TREU927/4 exhibits some resistance to lysis by normal human serum (NHS), but this resistance is never complete even after selection. The genome of this strain contains a minimum of eight sequences related to the T. brucei rhodesiense-specific serum resistance-associated gene (SRA), which encodes a truncated variant surface glycoprotein (VSG) conferring full resistance to lysis by NHS. We selected two sequences showing the highest similarity to SRA and also preceded by a region ("cotransposed region") present immediately upstream from SRA in the VSG expression site termed R-ES, where SRA is expressed in T. brucei rhodesiense. Whereas one of these sequences appears to be a pseudogene, the other, which is contained within a cluster of VSG basic copies (BCs), encodes a VSG truncated in the C-terminal domain. In the latter gene, an inserted region encoding surface-exposed loops similar to those of the BoTat 1.20 VSG interrupts the full SRA sequence. Therefore, this gene was termed SRA-BC, for the putative VSG basic copy from which SRA was derived. Neither this gene nor other SRA-like sequences appeared to be responsible for the relative resistance of TREU927/4 to NHS, since (i) transfection of SRA-BC in T. brucei brucei did not confer increased resistance; (ii) SRA transcripts could not be detected in this strain, even when focusing the search on the limited SRA sequence necessary to confer resistance and when using strain-specific SRA probes on RNA from cells selected in the presence of NHS.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
May
pubmed:issn
0166-6851
pubmed:author
pubmed:issnType
Print
pubmed:volume
135
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
39-47
pubmed:dateRevised
2010-11-18
pubmed:meshHeading
pubmed-meshheading:15287585-Amino Acid Sequence, pubmed-meshheading:15287585-Animals, pubmed-meshheading:15287585-Cattle, pubmed-meshheading:15287585-Conserved Sequence, pubmed-meshheading:15287585-Gene Order, pubmed-meshheading:15287585-Genes, Protozoan, pubmed-meshheading:15287585-Humans, pubmed-meshheading:15287585-Hydrophobic and Hydrophilic Interactions, pubmed-meshheading:15287585-Membrane Glycoproteins, pubmed-meshheading:15287585-Molecular Sequence Data, pubmed-meshheading:15287585-Multigene Family, pubmed-meshheading:15287585-Phenotype, pubmed-meshheading:15287585-Protein Sorting Signals, pubmed-meshheading:15287585-Protozoan Proteins, pubmed-meshheading:15287585-Sequence Alignment, pubmed-meshheading:15287585-Sequence Homology, Amino Acid, pubmed-meshheading:15287585-Serum, pubmed-meshheading:15287585-Trypanosoma brucei brucei, pubmed-meshheading:15287585-Trypanosoma brucei rhodesiense, pubmed-meshheading:15287585-Variant Surface Glycoproteins, Trypanosoma
pubmed:year
2004
pubmed:articleTitle
The Trypanosoma brucei reference strain TREU927/4 contains T. brucei rhodesiense-specific SRA sequences, but displays a distinct phenotype of relative resistance to human serum.
pubmed:affiliation
Laboratory of Molecular Parasitology, Department of Molecular Biology, IBMM, Free University of Brussels, 12, rue des Professeurs Jeener et Brachet, B-6041 Gosselies, Belgium.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't