Linked Life Data

15284494

Source:http://linkedlifedata.com/resource/pubmed/id/15284494

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
2004-7-30
pubmed:databankReference
pubmed:abstractText
Ethylene governs a range of developmental and response processes in plants. In Arabidopsis thaliana, the Raf-like kinase CTR1 acts as a key negative regulator of ethylene responses. While only one gene with CTR1 function apparently exists in Arabidopsis, we have isolated a family of CTR1- like genes in tomato ( Lycopersicon esculentum ). Based on amino acid alignments and phylogenetic analysis, these tomato CTR1- like genes are more similar to Arabidopsis CTR1 than any other sequences in the Arabidopsis genome. Structural analysis reveals considerable conservation in the size and position of the exons between Arabidopsis and tomato CTR1 genomic sequences. Complementation of the Arabidopsis ctr1-8 mutant with each of the tomato CTR genes indicates that they are all capable of functioning as negative regulators of the ethylene pathway. We previously reported that LeCTR1 expression is up-regulated in response to ethylene. Here, quantitative real-time PCR was carried out to detail expression for LeCTR1 and the additional CTR1 -like genes of tomato. Our results indicate that the tomato CTR1 gene family is differentially regulated at the mRNA level by ethylene and during stages of development marked by increased ethylene biosynthesis, including fruit ripening. The possibility of a multi-gene family of CTR1 -like genes in other species besides tomato was examined through mining of EST and genomic sequence databases.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Feb
pubmed:issn
0167-4412
pubmed:author
pubmed:issnType
Print
pubmed:volume
54
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
387-404
pubmed:dateRevised
2009-11-19
pubmed:meshHeading
pubmed-meshheading:15284494-Amino Acid Sequence, pubmed-meshheading:15284494-Arabidopsis, pubmed-meshheading:15284494-Cloning, Molecular, pubmed-meshheading:15284494-DNA, Complementary, pubmed-meshheading:15284494-DNA, Plant, pubmed-meshheading:15284494-Ethylenes, pubmed-meshheading:15284494-Exons, pubmed-meshheading:15284494-Gene Expression Regulation, Plant, pubmed-meshheading:15284494-Genes, Plant, pubmed-meshheading:15284494-Genetic Complementation Test, pubmed-meshheading:15284494-Introns, pubmed-meshheading:15284494-Lycopersicon esculentum, pubmed-meshheading:15284494-Molecular Sequence Data, pubmed-meshheading:15284494-Multigene Family, pubmed-meshheading:15284494-Mutation, pubmed-meshheading:15284494-Phenotype, pubmed-meshheading:15284494-Phylogeny, pubmed-meshheading:15284494-Plant Growth Regulators, pubmed-meshheading:15284494-Plant Proteins, pubmed-meshheading:15284494-Plants, Genetically Modified, pubmed-meshheading:15284494-Protein Kinases, pubmed-meshheading:15284494-Sequence Analysis, DNA, pubmed-meshheading:15284494-Sequence Homology, Amino Acid, pubmed-meshheading:15284494-Signal Transduction
pubmed:year
2004
pubmed:articleTitle
Evidence that CTR1-mediated ethylene signal transduction in tomato is encoded by a multigene family whose members display distinct regulatory features.
pubmed:affiliation
Boyce Thompson Institute for Plant Research, Cornell University, Tower Road, Ithaca, NY 14853, USA.
pubmed:publicationType
Journal Article, Comparative Study, Research Support, U.S. Gov't, Non-P.H.S.