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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
41
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pubmed:dateCreated |
2004-10-6
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pubmed:abstractText |
Tumor suppressor p53 has been implicated in cell stress response and determines cell fate of either growth arrest or apoptosis. Heat shock proteins (Hsps) expressed under stress usually confer survival protection to the cell or interruption in the apoptotic pathways. Although Hsp90 can physically interact with p53, whether or not the hsp90 gene is influenced downstream of p53 in UV irradiation-induced apoptosis remains unclear. We have found that the level of p53 is elevated with the decline of Hsp90 in UV-irradiated cells and that malfunction of Hsp90, as inhibited by geldanamycin, enhances the p53-involved UV irradiation-induced apoptosis. In addition, the expression of the hsp90beta gene was reduced in both UV-irradiated and wild type p53-transfected cells. These results suggest a negative correlation between the trans factor p53 and a chaperone gene hsp90beta in apoptotic cells. Mutation analysis demonstrated that the p53 binding site in the first exon was indispensable for p53 regulation on the hsp90beta gene. In addition, with p53 bound at the promoter of the hsp90beta gene, mSin3a and p300 were differentially recruited in UV irradiation-treated or untreated Jurkat cells in vivo. The evidence of p53-repressed hsp90beta gene expression in UV-irradiated cells shed light on a novel pathway of Hsp90 in the survival control of the stressed cells.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antibiotics, Antineoplastic,
http://linkedlifedata.com/resource/pubmed/chemical/Benzoquinones,
http://linkedlifedata.com/resource/pubmed/chemical/Chloramphenicol O-Acetyltransferase,
http://linkedlifedata.com/resource/pubmed/chemical/DNA,
http://linkedlifedata.com/resource/pubmed/chemical/HSP90 Heat-Shock Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/HSP90AB1 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Lactams, Macrocyclic,
http://linkedlifedata.com/resource/pubmed/chemical/Quinones,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger,
http://linkedlifedata.com/resource/pubmed/chemical/Tumor Suppressor Protein p53,
http://linkedlifedata.com/resource/pubmed/chemical/geldanamycin
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pubmed:status |
MEDLINE
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pubmed:month |
Oct
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pubmed:issn |
0021-9258
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
8
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pubmed:volume |
279
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
42545-51
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pubmed:dateRevised |
2008-11-21
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pubmed:meshHeading |
pubmed-meshheading:15284248-Antibiotics, Antineoplastic,
pubmed-meshheading:15284248-Apoptosis,
pubmed-meshheading:15284248-Base Sequence,
pubmed-meshheading:15284248-Benzoquinones,
pubmed-meshheading:15284248-Binding Sites,
pubmed-meshheading:15284248-Blotting, Western,
pubmed-meshheading:15284248-Cell Nucleus,
pubmed-meshheading:15284248-Cell Separation,
pubmed-meshheading:15284248-Cell Survival,
pubmed-meshheading:15284248-Chloramphenicol O-Acetyltransferase,
pubmed-meshheading:15284248-DNA,
pubmed-meshheading:15284248-DNA Mutational Analysis,
pubmed-meshheading:15284248-Dose-Response Relationship, Drug,
pubmed-meshheading:15284248-Exons,
pubmed-meshheading:15284248-Flow Cytometry,
pubmed-meshheading:15284248-HSP90 Heat-Shock Proteins,
pubmed-meshheading:15284248-Humans,
pubmed-meshheading:15284248-Immunoprecipitation,
pubmed-meshheading:15284248-Jurkat Cells,
pubmed-meshheading:15284248-Lactams, Macrocyclic,
pubmed-meshheading:15284248-Models, Genetic,
pubmed-meshheading:15284248-Molecular Sequence Data,
pubmed-meshheading:15284248-Mutation,
pubmed-meshheading:15284248-Plasmids,
pubmed-meshheading:15284248-Point Mutation,
pubmed-meshheading:15284248-Promoter Regions, Genetic,
pubmed-meshheading:15284248-Protein Binding,
pubmed-meshheading:15284248-Quinones,
pubmed-meshheading:15284248-RNA, Messenger,
pubmed-meshheading:15284248-Time Factors,
pubmed-meshheading:15284248-Transfection,
pubmed-meshheading:15284248-Tumor Suppressor Protein p53,
pubmed-meshheading:15284248-Ultraviolet Rays
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pubmed:year |
2004
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pubmed:articleTitle |
Repression of hsp90beta gene by p53 in UV irradiation-induced apoptosis of Jurkat cells.
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pubmed:affiliation |
National Laboratory of Medical Molecular Biology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100005, China.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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