Linked Life Data

15284204

Source:http://linkedlifedata.com/resource/pubmed/id/15284204

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
11
pubmed:dateCreated
2004-10-18
pubmed:abstractText
Previous work suggests that normal GLUT4 content is sufficient for increases in muscle glucose uptake (MGU) during hyperinsulinemia, because glucose phosphorylation is the more formidable barrier to insulin-stimulated MGU. It was hypothesized that a partial ablation of GLUT4 would not impair insulin-stimulated MGU when glucose phosphorylation capacity is normal but would do so when glucose phosphorylation capacity is increased. Thus, chow-fed C57BL/6J mice with a GLUT4 partial knockout (GLUT4(+/-)), hexokinase II overexpression (HK(Tg)), or both (HK(Tg) + GLUT4(+/-)) and wild-type littermates were studied. Carotid artery and jugular vein catheters were implanted for sampling and infusions at 4 months of age. After a 5-d recovery, 5-h fasted mice (n = 8-11/group) underwent a 120-min saline infusion or insulin clamp (4 mU/kg.min insulin with glucose maintained at 165 mg/dl) and received a 2-deoxy[(3)H]glucose bolus to provide an index of MGU (R(g)) for the soleus, gastrocnemius, and superficial vastus lateralis. Basal R(g) from all muscles studied from saline-infused mice were not changed by any of the genetic modifications. HK(Tg) mice had augmented insulin-stimulated R(g) in all muscles studied compared with remaining genotypes. Insulin-stimulated R(g) was not impaired in any of the muscles studied from GLUT4(+/-) mice. However, the enhanced insulin-stimulated R(g) created by HK overexpression was ablated in HK(Tg) + GLUT4(+/-) mice. Thus, a 50% reduction of normal GLUT4 content in the presence of normal HK activity does not impair insulin-stimulated MGU. However, when the glucose phosphorylation barrier is lowered by HK overexpression, GLUT4 availability becomes a limitation to insulin-stimulated MGU.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
AIM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Nov
pubmed:issn
0013-7227
pubmed:author
pubmed:issnType
Print
pubmed:volume
145
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
4912-6
pubmed:dateRevised
2011-11-17
pubmed:meshHeading
pubmed-meshheading:15284204-Animals, pubmed-meshheading:15284204-Basal Metabolism, pubmed-meshheading:15284204-Blood Glucose, pubmed-meshheading:15284204-Consciousness, pubmed-meshheading:15284204-Female, pubmed-meshheading:15284204-Glucose Transporter Type 4, pubmed-meshheading:15284204-Hexokinase, pubmed-meshheading:15284204-Humans, pubmed-meshheading:15284204-Hypoglycemic Agents, pubmed-meshheading:15284204-Insulin, pubmed-meshheading:15284204-Male, pubmed-meshheading:15284204-Mice, pubmed-meshheading:15284204-Mice, Inbred C57BL, pubmed-meshheading:15284204-Mice, Knockout, pubmed-meshheading:15284204-Mice, Transgenic, pubmed-meshheading:15284204-Monosaccharide Transport Proteins, pubmed-meshheading:15284204-Muscle, Skeletal, pubmed-meshheading:15284204-Muscle Proteins, pubmed-meshheading:15284204-Phosphorylation
pubmed:year
2004
pubmed:articleTitle
Regulation of insulin-stimulated muscle glucose uptake in the conscious mouse: role of glucose transport is dependent on glucose phosphorylation capacity.
pubmed:affiliation
Duke University Medical Center, Department of Pharmacology and Cancer Biology, 4321 Medical Park Drive, Suite 200, Durham, North Carolina 27704, USA. patrick.fueger@duke.edu
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S.