Linked Life Data

15282746

Source:http://linkedlifedata.com/resource/pubmed/id/15282746

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
2004-7-29
pubmed:abstractText
The entry of beta-catenin into vegetal cell nuclei beginning at the 16-cell stage is one of the earliest known molecular asymmetries seen along the animal-vegetal axis in the sea urchin embryo. Nuclear beta-catenin activates a vegetal signaling cascade that mediates micromere specification and specification of the endomesoderm in the remaining cells of the vegetal half of the embryo. Only a few potential target genes of nuclear beta-catenin have been functionally analyzed in the sea urchin embryo. Here, we show that SpWnt8, a Wnt8 homolog from Strongylocentrotus purpuratus, is zygotically activated specifically in 16-cell-stage micromeres in a nuclear beta-catenin-dependent manner, and its expression remains restricted to the micromeres until the 60-cell stage. At the late 60-cell stage nuclear beta-catenin-dependent SpWnt8 expression expands to the veg2 cell tier. SpWnt8 is the only signaling molecule thus far identified with expression localized to the 16-60-cell stage micromeres and the veg2 tier. Overexpression of SpWnt8 by mRNA microinjection produced embryos with multiple invagination sites and showed that, consistent with its localization, SpWnt8 is a strong inducer of endoderm. Blocking SpWnt8 function using SpWnt8 morpholino antisense oligonucleotides produced embryos that formed micromeres that could transmit the early endomesoderm-inducing signal, but these cells failed to differentiate as primary mesenchyme cells. SpWnt8-morpholino embryos also did not form endoderm, or secondary mesenchyme-derived pigment and muscle cells, indicating a role for SpWnt8 in gastrulation and in the differentiation of endomesodermal lineages. These results establish SpWnt8 as a critical component of the endomesoderm regulatory network in the sea urchin embryo.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jul
pubmed:issn
1526-954X
pubmed:author
pubmed:copyrightInfo
Copyright 2004 Wiley-Liss, Inc.
pubmed:issnType
Print
pubmed:volume
39
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
194-205
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed-meshheading:15282746-Animals, pubmed-meshheading:15282746-Blotting, Northern, pubmed-meshheading:15282746-Cell Differentiation, pubmed-meshheading:15282746-Cell Lineage, pubmed-meshheading:15282746-Cell Nucleus, pubmed-meshheading:15282746-Cytoskeletal Proteins, pubmed-meshheading:15282746-DNA Primers, pubmed-meshheading:15282746-Endoderm, pubmed-meshheading:15282746-Fluorescent Antibody Technique, pubmed-meshheading:15282746-Gastrula, pubmed-meshheading:15282746-Gene Expression Profiling, pubmed-meshheading:15282746-Gene Expression Regulation, Developmental, pubmed-meshheading:15282746-In Situ Hybridization, pubmed-meshheading:15282746-Mesoderm, pubmed-meshheading:15282746-Microinjections, pubmed-meshheading:15282746-Oligonucleotides, Antisense, pubmed-meshheading:15282746-Plasmids, pubmed-meshheading:15282746-Proteins, pubmed-meshheading:15282746-Reverse Transcriptase Polymerase Chain Reaction, pubmed-meshheading:15282746-Sea Urchins, pubmed-meshheading:15282746-Signal Transduction, pubmed-meshheading:15282746-Trans-Activators, pubmed-meshheading:15282746-Wnt Proteins, pubmed-meshheading:15282746-Zebrafish Proteins, pubmed-meshheading:15282746-beta Catenin
pubmed:year
2004
pubmed:articleTitle
Nuclear beta-catenin-dependent Wnt8 signaling in vegetal cells of the early sea urchin embryo regulates gastrulation and differentiation of endoderm and mesodermal cell lineages.
pubmed:affiliation
Department of Zoology, University of Hawaii at Manoa, Honolulu, Hawaii 96822, USA. athula@hawaii.edu
pubmed:publicationType
Journal Article, Comparative Study, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't