Source:http://linkedlifedata.com/resource/pubmed/id/15281797
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
30
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pubmed:dateCreated |
2004-7-29
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pubmed:abstractText |
We have exploited the saturation difference (SD) NMR technique to investigate the mutarotations of two sugars, l-fucose and d-ribose, at equilibrium. The KM and kcat values of Escherichia coli FucU-catalyzed mutarotation of l-fucose have been determined by this technique, and the values of KM for alpha- and beta-forms are shown to be the same. Similarly, the spontaneous mutarotation of d-ribose was investigated by using the same method, and it was found that d-ribose has an exceptionally high spontaneous alpha-to-beta conversion rate only between the furan forms.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Carbohydrate Epimerases,
http://linkedlifedata.com/resource/pubmed/chemical/Fucose,
http://linkedlifedata.com/resource/pubmed/chemical/Furans,
http://linkedlifedata.com/resource/pubmed/chemical/Pyrans,
http://linkedlifedata.com/resource/pubmed/chemical/aldose 1-epimerase
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pubmed:status |
MEDLINE
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pubmed:month |
Aug
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pubmed:issn |
0002-7863
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:day |
4
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pubmed:volume |
126
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
9180-1
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pubmed:dateRevised |
2008-1-17
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pubmed:meshHeading |
pubmed-meshheading:15281797-Carbohydrate Conformation,
pubmed-meshheading:15281797-Carbohydrate Epimerases,
pubmed-meshheading:15281797-Catalysis,
pubmed-meshheading:15281797-Fucose,
pubmed-meshheading:15281797-Furans,
pubmed-meshheading:15281797-Kinetics,
pubmed-meshheading:15281797-Nuclear Magnetic Resonance, Biomolecular,
pubmed-meshheading:15281797-Pyrans,
pubmed-meshheading:15281797-Stereoisomerism
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pubmed:year |
2004
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pubmed:articleTitle |
NMR analysis of enzyme-catalyzed and free-equilibrium mutarotation kinetics of monosaccharides.
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pubmed:affiliation |
Yusong-Gu, Gusong-Dong 373-1, Department of Chemistry and National Creative Research Institute Center, Korea Advanced Institute of Science and Technology, Daejon 305-701, South Korea.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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