Linked Life Data

15280585

Source:http://linkedlifedata.com/resource/pubmed/id/15280585

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:dateCreated
2004-7-28
pubmed:abstractText
Immortalized chondrocytes of human origin have been developed to serve as reproducible models for studying chondrocyte function. In this chapter, methods for immortalization of primary human chondrocytes with SV40-TAg, HPV-16 E6/E7, and telomerase by retrovirally mediated transduction and selection for neomycin resistance are described. However, stable integration of an immortalizing gene stabilizes proliferative capacity, but not the differentiated chondrocyte phenotype. Thus, strategies for selection of chondrocyte cell lines, involving the maintenance of high cell density and moderation of cell proliferation, are also described. The methods for immortalization and selection are applicable to the development of chondrocyte cell lines using any immortalizing agent. Although immortalized chondrocytes should not be considered as substitutes for primary chondrocytes, they may be useful tools for evaluating and further validating mechanisms relevant to cartilage biology.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
1543-1894
pubmed:author
pubmed:issnType
Print
pubmed:volume
100
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
23-36
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed:year
2004
pubmed:articleTitle
Immortalization of human articular chondrocytes for generation of stable, differentiated cell lines.
pubmed:affiliation
Division of Rheumatology, Beth Israel Deaconess Medical Center, New England Baptist Bone and Joint Institute, Harvard Institutes of Medicine, Boston, MA, USA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't