Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
Pt 4
pubmed:dateCreated
2004-7-28
pubmed:databankReference
pubmed:abstractText
A taxonomic study was performed on 26 strains isolated from microbial mats in Antarctic lakes of the Vestfold Hills and the McMurdo Dry Valleys. Phylogenetic analysis based on 16S rRNA gene sequences placed these strains within the Rhodobacter group of the alpha-subclass of the Proteobacteria. Sequence similarity values for the strains with their nearest phylogenetic neighbours (Jannaschia, Octadecabacter and Ketogulonicigenium) ranged between 94.0 and 95.8%. DNA-DNA hybridizations and comparison of repetitive extragenic palindromic DNA-PCR (rep-PCR) fingerprinting patterns revealed that these strains are members of three distinct species. The isolates are Gram-negative, chemoheterotrophic, non-motile rods and their DNA G+C contents range from 59.4 to 66.4 mol%. Whole-cell fatty acid profiles are similar and the primary fatty acid in all the strains is 18 : 1 omega7c (74.1-87.7% of total). Genotypic results together with phenotypic characteristics allowed the differentiation of these species from related recognized species of the alpha-Proteobacteria and the strains are assigned to a new genus, Loktanella gen. nov., with three novel species: Loktanella salsilacus sp. nov. (type species), consisting of ten strains with LMG 21507T (=CIP 108322T) as type strain; Loktanella fryxellensis sp. nov., consisting of 12 strains with LMG 22007T (=CIP 108323T) as type strain; and Loktanella vestfoldensis sp. nov., consisting of four strains with LMG 22003T (=CIP 108321TT) as type strain.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jul
pubmed:issn
1466-5026
pubmed:author
pubmed:issnType
Print
pubmed:volume
54
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1263-9
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed-meshheading:15280301-Antarctic Regions, pubmed-meshheading:15280301-Bacterial Typing Techniques, pubmed-meshheading:15280301-Base Composition, pubmed-meshheading:15280301-DNA, Bacterial, pubmed-meshheading:15280301-DNA, Ribosomal, pubmed-meshheading:15280301-DNA Fingerprinting, pubmed-meshheading:15280301-Fatty Acids, pubmed-meshheading:15280301-Fresh Water, pubmed-meshheading:15280301-Genes, rRNA, pubmed-meshheading:15280301-Gentian Violet, pubmed-meshheading:15280301-Geologic Sediments, pubmed-meshheading:15280301-Molecular Sequence Data, pubmed-meshheading:15280301-Movement, pubmed-meshheading:15280301-Nucleic Acid Hybridization, pubmed-meshheading:15280301-Phenazines, pubmed-meshheading:15280301-Phylogeny, pubmed-meshheading:15280301-RNA, Bacterial, pubmed-meshheading:15280301-RNA, Ribosomal, 16S, pubmed-meshheading:15280301-Repetitive Sequences, Nucleic Acid, pubmed-meshheading:15280301-Rhodobacteraceae, pubmed-meshheading:15280301-Sequence Analysis, DNA, pubmed-meshheading:15280301-Sequence Homology, pubmed-meshheading:15280301-Water Microbiology
pubmed:year
2004
pubmed:articleTitle
Loktanella salsilacus gen. nov., sp. nov., Loktanella fryxellensis sp. nov. and Loktanella vestfoldensis sp. nov., new members of the Rhodobacter group, isolated from microbial mats in Antarctic lakes.
pubmed:affiliation
Laboratorium voor Microbiologie, Vakgroep Biochemie, Fysiologie en Microbiologie, Universiteit Gent, K. L. Ledeganckstraat 35, B-9000 Gent, Belgium. stefanie.vantrappen@UGent.be
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't