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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
7
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pubmed:dateCreated |
2004-7-27
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pubmed:databankReference |
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pubmed:abstractText |
We have isolated the Penicillium chrysogenum pex5 gene encoding the receptor for microbody matrix proteins containing a type 1 peroxisomal targeting signal (PTS1). Pc-pex5 contains 2 introns and encodes a protein of approximately 75 kDa. P. chrysogenum pex5 disruptants appear to be highly unstable, show poor growth, and are unable to sporulate asexually. Furthermore, pex5 cells mislocalize a fluorescent PTS1 reporter protein to the cytosol. Pc-pex5 was expressed in a PEX5 null mutant of the yeast Hansenula polymorpha. Detailed analysis demonstrated that the PTS1 proteins dihydroxyacetone synthase and catalase were almost fully imported into microbodies. Surprisingly, alcohol oxidase, which also depends on Pex5p for import into microbodies, remained mainly in the cytosol. Thus, P. chrysogenum Pex5p has a different specificity of cargo recognition than its H. polymorpha counterpart. This was also suggested by the observation that Pc-Pex5p sorted a reporter protein fused to various functional PTS1 signals with different efficiencies.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Alcohol Oxidoreductases,
http://linkedlifedata.com/resource/pubmed/chemical/Aldehyde-Ketone Transferases,
http://linkedlifedata.com/resource/pubmed/chemical/Catalase,
http://linkedlifedata.com/resource/pubmed/chemical/DNA, Complementary,
http://linkedlifedata.com/resource/pubmed/chemical/DNA, Fungal,
http://linkedlifedata.com/resource/pubmed/chemical/Fungal Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Cytoplasmic and Nuclear,
http://linkedlifedata.com/resource/pubmed/chemical/Recombinant Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/alcohol oxidase,
http://linkedlifedata.com/resource/pubmed/chemical/formaldehyde transketolase,
http://linkedlifedata.com/resource/pubmed/chemical/peroxisome-targeting signal 1...
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pubmed:status |
MEDLINE
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pubmed:month |
Jul
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pubmed:issn |
1087-1845
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
41
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
708-20
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:15275666-Alcohol Oxidoreductases,
pubmed-meshheading:15275666-Aldehyde-Ketone Transferases,
pubmed-meshheading:15275666-Amino Acid Sequence,
pubmed-meshheading:15275666-Catalase,
pubmed-meshheading:15275666-Conserved Sequence,
pubmed-meshheading:15275666-Cytoplasm,
pubmed-meshheading:15275666-DNA, Complementary,
pubmed-meshheading:15275666-DNA, Fungal,
pubmed-meshheading:15275666-Fungal Proteins,
pubmed-meshheading:15275666-Gene Deletion,
pubmed-meshheading:15275666-Genes, Fungal,
pubmed-meshheading:15275666-Introns,
pubmed-meshheading:15275666-Microbodies,
pubmed-meshheading:15275666-Molecular Sequence Data,
pubmed-meshheading:15275666-Molecular Weight,
pubmed-meshheading:15275666-Mutagenesis, Insertional,
pubmed-meshheading:15275666-Penicillium chrysogenum,
pubmed-meshheading:15275666-Pichia,
pubmed-meshheading:15275666-Protein Transport,
pubmed-meshheading:15275666-Receptors, Cytoplasmic and Nuclear,
pubmed-meshheading:15275666-Recombinant Proteins,
pubmed-meshheading:15275666-Sequence Alignment,
pubmed-meshheading:15275666-Spores, Fungal
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pubmed:year |
2004
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pubmed:articleTitle |
Penicillium chrysogenum Pex5p mediates differential sorting of PTS1 proteins to microbodies of the methylotrophic yeast Hansenula polymorpha.
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pubmed:affiliation |
Eukaryotic Microbiology, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, P.O. Box 14, Haren NL-9750 AA, The Netherlands. j.a.k.w.kiel@biol.rug.nl
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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