Linked Life Data

15274090

Source:http://linkedlifedata.com/resource/pubmed/id/15274090

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
6
pubmed:dateCreated
2004-7-26
pubmed:abstractText
Fluorescein-labeled antibodies are widely used in clinical assays and fluorescence microscopy. The fluorescent signal per labeled antibody is limited by fluorescein self-quenching, which occurs when the antibody is heavily labeled with multiple fluoresceins. We examined immunoglobulin G (IgG) when labeled with 0.7 to about 30 fluoresceins per antibody molecule. The extent of self-quenching was decreased, and the signal increased, when the labeled antibody was in close proximity to metallic silver particles. Time-resolved measurements showed that the intensity increase was due in part to a silver-induced increase in the radiative decay rate. These results suggest the use of labeled antibodies conjugated to silver particles as ultrabright probes for imaging or analytical applications.
pubmed:grant
pubmed:commentsCorrections
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Aug
pubmed:issn
0006-3525
pubmed:author
pubmed:issnType
Print
pubmed:day
15
pubmed:volume
74
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
467-75
pubmed:dateRevised
2011-5-17
pubmed:meshHeading
pubmed:year
2004
pubmed:articleTitle
Ultrabright fluorescein-labeled antibodies near silver metallic surfaces.
pubmed:affiliation
Center for Fluorescence Spectroscopy, University of Maryland at Baltimore, Department of Biochemistry, 725 West Lombard Street, Baltimore, MD 21201, USA. lakowicz@cfs.umbi.umd.edu
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S.