Linked Life Data

1527097

Source:http://linkedlifedata.com/resource/pubmed/id/1527097

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
6
pubmed:dateCreated
1992-10-22
pubmed:abstractText
A parallel-plate flow chamber is developed in order to study cellular adhesion phenomena. An image analysis system is used to observe individual cells exposed to flow in situ and to determine area, perimeter, and shape of these cells as a function of time and shear stress. With this flow system the behavior of human fibroblasts spread on glass is studied when exposed to an increasing laminar flow. The flow system appears to be well-suited for following individual cells during detachment. After 75 to 90 min, at a shear stress of 350 dynes/cm2, more than 50% of the spread cells are detached from the surface. Cells with higher spreading areas stay longer at the glass surface. Cells round up before detaching. Sometimes the cell body is attached to the substratum through a thin filament during detachment. At the scanning electron microscopy level numerous filopodial extensions are observed. Cell material could only rarely be observed at the light or scanning electron microscopic level on the substratum once a cell was detached.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:status
MEDLINE
pubmed:month
Jun
pubmed:issn
0021-9304
pubmed:author
pubmed:issnType
Print
pubmed:volume
26
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
725-38
pubmed:dateRevised
2008-11-21
pubmed:meshHeading
pubmed:year
1992
pubmed:articleTitle
Development and use of a parallel-plate flow chamber for studying cellular adhesion to solid surfaces.
pubmed:affiliation
Laboratory for Materia Technica, University of Groningen, The Netherlands.
pubmed:publicationType
Journal Article