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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
3
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pubmed:dateCreated |
1992-10-19
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pubmed:databankReference |
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pubmed:abstractText |
We previously described the isolation of three identical complementary DNA (cDNA) clones, constructed from Orchard/Cocksfoot grass (Dactylis glomerata) anther messenger RNA (mRNA), expressing a 140,000 MW beta-galactosidase fusion protein recognized by IgE antibodies in atopic sera. Partial nucleotide sequencing and inferred amino acid sequence showed greater than 90% homology with the group II allergen from Lolium perenne (Lol II) indicating they encode the group II equivalent, Dac g II. Western blot immunoprobing of recombinant lysates with rabbit polyclonal, mouse monoclonal and human polyclonal antisera demonstrates immunological identity between recombinant Dac g II, Lol p I and Lol p II. Similar cross-identity is observed with pollen extracts from three other grass species: Festuca rubra, Phleum pratense and Anthoxanthum odoratum. Recombinant Dac g II was recognized by species- and group-cross-reactive human IgE antibodies in 33% (4/12) of sera randomly selected from grass-sensitive individuals and in 67% (14/21) of sera from patients receiving grass pollen immunotherapy, whilst 0/4 sera from patients receiving venom immunotherapy alone contained Dac g II cross-reactive IgE. Cross-reactive IgG4 antibodies were detectable in 95% of sera from grass pollen immunotherapy patients. These preliminary data suggest that conventional grass pollen allergoid desensitization immunotherapy may induce IgE responses to a cross-reactive epitope(s) co-expressed by grass pollen groups I and II (and possibly group III) allergens.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/1526648-1671715,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1526648-1693910,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1526648-1697854,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1526648-1827749,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1526648-1863893,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1526648-2210872,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1526648-2210876,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1526648-2411668,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1526648-2414230,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1526648-2422284,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1526648-2434551,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1526648-2434841,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1526648-2460536,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1526648-2462587,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1526648-2472390,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1526648-2523360,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1526648-2605214,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1526648-2759718,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1526648-2807580,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1526648-2913133,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1526648-3492310,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1526648-3518747,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1526648-3693899,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1526648-3893674,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1526648-4192674,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1526648-6194195,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1526648-6600252,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1526648-7041130,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1526648-889700
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jul
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pubmed:issn |
0019-2805
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
76
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
389-96
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pubmed:dateRevised |
2010-11-18
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pubmed:meshHeading |
pubmed-meshheading:1526648-Allergens,
pubmed-meshheading:1526648-Amino Acid Sequence,
pubmed-meshheading:1526648-Antigens, Plant,
pubmed-meshheading:1526648-Base Sequence,
pubmed-meshheading:1526648-Blotting, Western,
pubmed-meshheading:1526648-Chromatography, Affinity,
pubmed-meshheading:1526648-Cross Reactions,
pubmed-meshheading:1526648-Humans,
pubmed-meshheading:1526648-Hypersensitivity,
pubmed-meshheading:1526648-Immunoglobulin E,
pubmed-meshheading:1526648-Immunoglobulin G,
pubmed-meshheading:1526648-Immunotherapy,
pubmed-meshheading:1526648-Molecular Sequence Data,
pubmed-meshheading:1526648-Plant Proteins,
pubmed-meshheading:1526648-Poaceae,
pubmed-meshheading:1526648-Pollen,
pubmed-meshheading:1526648-Radioallergosorbent Test,
pubmed-meshheading:1526648-Recombinant Fusion Proteins,
pubmed-meshheading:1526648-Sequence Homology, Nucleic Acid
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pubmed:year |
1992
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pubmed:articleTitle |
Recombinant pollen allergens from Dactylis glomerata: preliminary evidence that human IgE cross-reactivity between Dac g II and Lol p I/II is increased following grass pollen immunotherapy.
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pubmed:affiliation |
University Department of Clinical Biochemistry, Queen Elizabeth Medical Center, Birmingham, U.K.
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pubmed:publicationType |
Journal Article,
In Vitro
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