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rdf:type |
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lifeskim:mentions |
umls-concept:C0002085,
umls-concept:C0019721,
umls-concept:C0026926,
umls-concept:C0030956,
umls-concept:C0039194,
umls-concept:C0085358,
umls-concept:C0086418,
umls-concept:C0439064,
umls-concept:C0449255,
umls-concept:C1332714,
umls-concept:C1332717,
umls-concept:C1413244,
umls-concept:C1706438,
umls-concept:C1880022,
umls-concept:C2698600
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pubmed:issue |
3
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pubmed:dateCreated |
2004-7-21
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pubmed:abstractText |
The secreted Mycobacterium tuberculosis 10-kDa culture filtrate protein (CFP)10 is a potent T cell Ag that is recognized by a high percentage of persons infected with M. tuberculosis. We determined the molecular basis for this widespread recognition by identifying and characterizing a 15-mer peptide, CFP10(71-85), that elicited IFN-gamma production and CTL activity by both CD4(+) and CD8(+) T cells from persons expressing multiple MHC class II and class I molecules, respectively. CFP10(71-85) contained at least two epitopes, one of 10 aa (peptide T1) and another of 9 aa (peptide T6). T1 was recognized by CD4(+) cells in the context of DRB1*04, DR5*0101, and DQB1*03, and by CD8(+) cells of A2(+) donors. T6 elicited responses by CD4(+) cells in the context of DRB1*04 and DQB1*03, and by CD8(+) cells of B35(+) donors. Deleting a single amino acid from the amino or carboxy terminus of either peptide markedly reduced IFN-gamma production, suggesting that they are minimal epitopes for both CD4(+) and CD8(+) cells. As far as we are aware, these are the shortest microbial peptides that have been found to elicit responses by both T cell subpopulations. The capacity of CFP10(71-85) to stimulate IFN-gamma production and CTL activity by CD4(+) and CD8(+) cells from persons expressing a spectrum of MHC molecules suggests that this peptide is an excellent candidate for inclusion in a subunit antituberculosis vaccine.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
AIM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, Bacterial,
http://linkedlifedata.com/resource/pubmed/chemical/Bacterial Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/CFP-10 protein, Mycobacterium...,
http://linkedlifedata.com/resource/pubmed/chemical/Epitopes,
http://linkedlifedata.com/resource/pubmed/chemical/HLA Antigens,
http://linkedlifedata.com/resource/pubmed/chemical/HLA-DQ Antigens,
http://linkedlifedata.com/resource/pubmed/chemical/HLA-DQ beta-Chains,
http://linkedlifedata.com/resource/pubmed/chemical/HLA-DQB1 antigen,
http://linkedlifedata.com/resource/pubmed/chemical/HLA-DR Antigens,
http://linkedlifedata.com/resource/pubmed/chemical/HLA-DRB1 Chains,
http://linkedlifedata.com/resource/pubmed/chemical/Interferon-gamma,
http://linkedlifedata.com/resource/pubmed/chemical/Peptide Fragments,
http://linkedlifedata.com/resource/pubmed/chemical/Tuberculosis Vaccines
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pubmed:status |
MEDLINE
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pubmed:month |
Aug
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pubmed:issn |
0022-1767
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pubmed:author |
pubmed-author:AndersenPeterP,
pubmed-author:BarnesPeter FPF,
pubmed-author:EwerKatieK,
pubmed-author:KlucarPeterP,
pubmed-author:LalvaniAjitA,
pubmed-author:LewinsohnDavid MDM,
pubmed-author:MoonanPatrick KPK,
pubmed-author:NepomGerald TGT,
pubmed-author:SafiHassanH,
pubmed-author:ShamsHomayounH,
pubmed-author:WeisSteven ESE,
pubmed-author:WizelBenjaminB
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pubmed:issnType |
Print
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pubmed:day |
1
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pubmed:volume |
173
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1966-77
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pubmed:dateRevised |
2011-11-17
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pubmed:meshHeading |
pubmed-meshheading:15265931-Alleles,
pubmed-meshheading:15265931-Amino Acid Substitution,
pubmed-meshheading:15265931-Antigens, Bacterial,
pubmed-meshheading:15265931-Bacterial Proteins,
pubmed-meshheading:15265931-CD4-Positive T-Lymphocytes,
pubmed-meshheading:15265931-CD8-Positive T-Lymphocytes,
pubmed-meshheading:15265931-Cytotoxicity, Immunologic,
pubmed-meshheading:15265931-Epitopes,
pubmed-meshheading:15265931-HLA Antigens,
pubmed-meshheading:15265931-HLA-DQ Antigens,
pubmed-meshheading:15265931-HLA-DQ beta-Chains,
pubmed-meshheading:15265931-HLA-DR Antigens,
pubmed-meshheading:15265931-HLA-DRB1 Chains,
pubmed-meshheading:15265931-Humans,
pubmed-meshheading:15265931-Interferon-gamma,
pubmed-meshheading:15265931-Mycobacterium tuberculosis,
pubmed-meshheading:15265931-Peptide Fragments,
pubmed-meshheading:15265931-Sequence Deletion,
pubmed-meshheading:15265931-T-Cell Antigen Receptor Specificity,
pubmed-meshheading:15265931-Tuberculosis Vaccines
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pubmed:year |
2004
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pubmed:articleTitle |
Characterization of a Mycobacterium tuberculosis peptide that is recognized by human CD4+ and CD8+ T cells in the context of multiple HLA alleles.
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pubmed:affiliation |
Center for Pulmonary and Infectious Disease Control, and Department of Microbiology, University of Texas Health Center, Tyler, TX 75708, USA. amir.shams@uthct.edu
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pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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