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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
41
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pubmed:dateCreated |
2004-10-6
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pubmed:abstractText |
The early growth response-1 transcription factor (Egr-1) is induced as part of the immediate-early gene expression response during early liver regeneration. In the studies reported here the functional significance of EGR-1 expression during liver regeneration was examined by characterizing the hepatic regenerative response to partial hepatectomy in Egr-1 null mice. The results of these studies showed that liver regeneration in Egr-1 null mice is impaired. Although activation of interleukin-6-STAT3 signaling, regulation of expression of hepatic C/ebpalpha, C/ebpbeta, cyclin D, and cyclin E and progression through the first wave of hepatocellular DNA synthesis occurred appropriately following partial hepatectomy in Egr-1 null mice, subsequent signaling events and cell cycle progression after the first round of DNA synthesis were deranged. This derangement was characterized by increased activation of the p38 mitogen-activated protein kinase and inhibition of hepatocellular metaphase-to-anaphase mitotic progression. Together these observations suggest that EGR-1 is an important regulator of hepatocellular mitotic progression. In support of this, microarray-based gene expression analysis showed that induction of expression of the cell division cycle 20 gene (Cdc20), a key regulator of the mitotic anaphase-promoting complex, is significantly reduced in Egr-1 null mice. Taken together these data define a novel functional role for EGR-1 in regulating hepatocellular mitotic progression through the spindle assembly checkpoint during liver regeneration.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Bromodeoxyuridine,
http://linkedlifedata.com/resource/pubmed/chemical/Cdc20 protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/Cell Cycle Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Coloring Agents,
http://linkedlifedata.com/resource/pubmed/chemical/Cyclin D,
http://linkedlifedata.com/resource/pubmed/chemical/Cyclin E,
http://linkedlifedata.com/resource/pubmed/chemical/Cyclins,
http://linkedlifedata.com/resource/pubmed/chemical/DNA,
http://linkedlifedata.com/resource/pubmed/chemical/DNA, Complementary,
http://linkedlifedata.com/resource/pubmed/chemical/DNA-Binding Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Early Growth Response Protein 1,
http://linkedlifedata.com/resource/pubmed/chemical/Immediate-Early Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Interleukin-6,
http://linkedlifedata.com/resource/pubmed/chemical/RNA,
http://linkedlifedata.com/resource/pubmed/chemical/STAT3 Transcription Factor,
http://linkedlifedata.com/resource/pubmed/chemical/Stat3 protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/Trans-Activators,
http://linkedlifedata.com/resource/pubmed/chemical/Transcription Factors,
http://linkedlifedata.com/resource/pubmed/chemical/p38 Mitogen-Activated Protein...
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pubmed:status |
MEDLINE
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pubmed:month |
Oct
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pubmed:issn |
0021-9258
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
8
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pubmed:volume |
279
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
43107-16
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pubmed:dateRevised |
2009-11-19
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pubmed:meshHeading |
pubmed-meshheading:15265859-Anaphase,
pubmed-meshheading:15265859-Animals,
pubmed-meshheading:15265859-Bromodeoxyuridine,
pubmed-meshheading:15265859-Cell Cycle,
pubmed-meshheading:15265859-Cell Cycle Proteins,
pubmed-meshheading:15265859-Cell Division,
pubmed-meshheading:15265859-Coloring Agents,
pubmed-meshheading:15265859-Cyclin D,
pubmed-meshheading:15265859-Cyclin E,
pubmed-meshheading:15265859-Cyclins,
pubmed-meshheading:15265859-DNA,
pubmed-meshheading:15265859-DNA, Complementary,
pubmed-meshheading:15265859-DNA-Binding Proteins,
pubmed-meshheading:15265859-Early Growth Response Protein 1,
pubmed-meshheading:15265859-Enzyme-Linked Immunosorbent Assay,
pubmed-meshheading:15265859-Gene Expression Regulation,
pubmed-meshheading:15265859-Immediate-Early Proteins,
pubmed-meshheading:15265859-Immunoblotting,
pubmed-meshheading:15265859-Interleukin-6,
pubmed-meshheading:15265859-Liver,
pubmed-meshheading:15265859-Metaphase,
pubmed-meshheading:15265859-Mice,
pubmed-meshheading:15265859-Mice, Inbred C57BL,
pubmed-meshheading:15265859-Mitosis,
pubmed-meshheading:15265859-Oligonucleotide Array Sequence Analysis,
pubmed-meshheading:15265859-RNA,
pubmed-meshheading:15265859-Regeneration,
pubmed-meshheading:15265859-Reverse Transcriptase Polymerase Chain Reaction,
pubmed-meshheading:15265859-STAT3 Transcription Factor,
pubmed-meshheading:15265859-Signal Transduction,
pubmed-meshheading:15265859-Time Factors,
pubmed-meshheading:15265859-Trans-Activators,
pubmed-meshheading:15265859-Transcription, Genetic,
pubmed-meshheading:15265859-Transcription Factors,
pubmed-meshheading:15265859-p38 Mitogen-Activated Protein Kinases
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pubmed:year |
2004
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pubmed:articleTitle |
Delayed hepatocellular mitotic progression and impaired liver regeneration in early growth response-1-deficient mice.
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pubmed:affiliation |
Department of Pediatrics, Washington University School of Medicine, St. Louis Children's Hospital, MO, USA.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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