Source:http://linkedlifedata.com/resource/pubmed/id/15265303
Switch to
Predicate | Object |
---|---|
rdf:type | |
lifeskim:mentions | |
pubmed:issue |
5-6
|
pubmed:dateCreated |
2004-7-21
|
pubmed:abstractText |
Studies have demonstrated the feasibility of transplanting cardiomyocytes after myocardial infarction (MI). However, persistence and effects on left ventricular (LV) function have not been elucidated in long-term studies. Ventricular fetal cardiomyocytes from embryos of both sexes were injected into marginal regions of MI 4 weeks after suture occlusion of the left anterior descending artery in adult female rats. Two and 6 months after transplantation (Tx), engrafted cells were traced by immunohistochemical in situ hybridization for Y chromosomes or bromodeoxyuridine (BrdU) staining, LV dimensions and function were assessed by echocardiography, and LV pressure was assessed ex vivo in a Langendorff perfusion system. Immunohistochemistry for alpha-sarcomeric actin and Y chromosomes revealed the presence of transplanted cells in infarcted host myocardium at both 2 and 6 months. End-diastolic LV diameter markedly decreased after Tx and fractional shortening gradually increased after Tx (31.3 +/- 4.5% before Tx, 45.4 +/- 4.2% at 6 months; p<0.005). Wall area fraction and MI size were unaffected by Tx. In hearts with MI, but not in normal hearts, Tx led to the development of higher pressures (87 +/- 18 versus 38 +/- 8 mmHg, 6 months post-Tx versus nontreated). After catecholamine stimulation, both infarcted and normal hearts developed higher pressures after Tx (p<0.005), ultimately associated with reduced mortality after Tx versus nontreated. Transplanted cardiomyocyte-rich graft cells persist in host myocardium and mediate continuous improvement of LV function and survival in a rat model of MI even during long-term follow-up, possibly involving a catecholamine-sensitive mechanism.
|
pubmed:language |
eng
|
pubmed:journal | |
pubmed:citationSubset |
IM
|
pubmed:status |
MEDLINE
|
pubmed:issn |
1076-3279
|
pubmed:author |
pubmed-author:BreuerSebastianS,
pubmed-author:FrankeAndreasA,
pubmed-author:GüntherKalleK,
pubmed-author:HanrathPeterP,
pubmed-author:LiehnElisa AEA,
pubmed-author:ReffelmannThorstenT,
pubmed-author:SchuhAlexanderA,
pubmed-author:SchwarzErnst RER,
pubmed-author:SkobelErikE,
pubmed-author:WeberChristianC
|
pubmed:issnType |
Print
|
pubmed:volume |
10
|
pubmed:owner |
NLM
|
pubmed:authorsComplete |
Y
|
pubmed:pagination |
849-64
|
pubmed:dateRevised |
2006-11-15
|
pubmed:meshHeading |
pubmed-meshheading:15265303-Animals,
pubmed-meshheading:15265303-Female,
pubmed-meshheading:15265303-Follow-Up Studies,
pubmed-meshheading:15265303-Longitudinal Studies,
pubmed-meshheading:15265303-Myocardial Infarction,
pubmed-meshheading:15265303-Myocytes, Cardiac,
pubmed-meshheading:15265303-Rats,
pubmed-meshheading:15265303-Rats, Sprague-Dawley,
pubmed-meshheading:15265303-Recovery of Function,
pubmed-meshheading:15265303-Survival Analysis,
pubmed-meshheading:15265303-Treatment Outcome,
pubmed-meshheading:15265303-Ventricular Dysfunction, Left
|
pubmed:articleTitle |
Transplantation of fetal cardiomyocytes into infarcted rat hearts results in long-term functional improvement.
|
pubmed:affiliation |
Medizinische Klinik I and Department of Molecular Cardiovascular Research, Department of Cardiology, University Hospital Aachen, Aachen, Germany. erik.skobel@t-online.de
|
pubmed:publicationType |
Journal Article,
Comparative Study,
Evaluation Studies
|