Source:http://linkedlifedata.com/resource/pubmed/id/15265288
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
5-6
|
| pubmed:dateCreated |
2004-7-21
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| pubmed:abstractText |
We investigated a portable bioartificial renal tubule device (BRTD) consisting of renal tubule cells and hollow fibers, to improve the quality of life of patients. It is necessary for a BRTD system to be compact. A compact portable BRTB requires transfection of an appropriate water channel or electrical pump genes in tubular epithelial cells, which should be based on physiological similarities to human kidney function. LLC-PK(1) cells, into which rat kidney aquaporin 1 (AQP1) cDNA was stably transfected, were evaluated for water transport ability. The expression and localization of water AQP1 were examined by Western blotting, RT-PCR, and immunofluorescence. To measure transcellular water permeation, a simple method was applied, using phenol red as a cell-impermeant marker of concentration. In contrast to wild-type LLC-PK(1) cells, rat AQP1-transfected cells had high transcellular osmotic water permeability. The expression of rat AQP1 mRNA (ratio of AQP1 to beta-actin mRNA) and protein bands (a 28-kDa band and a broad, 35- to 45-kDa band) was confirmed to be stably maintained until a population doubling level of 24. In AQP1-transfected LLCPK(1) cells, the protein was localized mainly to the basolateral side, but also the apical side, of the plasma membrane. Wild-type LLC-PK(1) cells were not stained at the plasma membrane. It is possible that enough AQP1-transfected tubule epithelial cells were supplied for a bioartificial renal tubule device.
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| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:chemical | |
| pubmed:status |
MEDLINE
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| pubmed:issn |
1076-3279
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| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
10
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| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
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| pubmed:pagination |
711-22
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| pubmed:dateRevised |
2006-11-15
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| pubmed:meshHeading |
pubmed-meshheading:15265288-Animals,
pubmed-meshheading:15265288-Aquaporins,
pubmed-meshheading:15265288-Bioartificial Organs,
pubmed-meshheading:15265288-Biological Transport, Active,
pubmed-meshheading:15265288-Cell Line,
pubmed-meshheading:15265288-Cell Membrane Permeability,
pubmed-meshheading:15265288-Feasibility Studies,
pubmed-meshheading:15265288-Gene Expression Regulation,
pubmed-meshheading:15265288-Genetic Enhancement,
pubmed-meshheading:15265288-Kidney, Artificial,
pubmed-meshheading:15265288-Kidney Tubules,
pubmed-meshheading:15265288-Protein Engineering,
pubmed-meshheading:15265288-Recombinant Proteins,
pubmed-meshheading:15265288-Swine,
pubmed-meshheading:15265288-Tissue Distribution,
pubmed-meshheading:15265288-Tissue Engineering,
pubmed-meshheading:15265288-Transfection,
pubmed-meshheading:15265288-Water,
pubmed-meshheading:15265288-Water-Electrolyte Balance
|
| pubmed:articleTitle |
Transcellular water transport and stability of expression in aquaporin 1-transfected LLC-PK1 cells in the development of a portable bioartificial renal tubule device.
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| pubmed:affiliation |
Division of Nephrology and Metabolism, Department of Medicine, Tokai University, Institute of Medical Sciences, Kanagawa, Japan.
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| pubmed:publicationType |
Journal Article,
Comparative Study,
Evaluation Studies
|