Linked Life Data

15264223

Source:http://linkedlifedata.com/resource/pubmed/id/15264223

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
2004-7-20
pubmed:abstractText
Human immunodeficiency virus type 1 (HIV-1)-associated dementia is observed in 20-30% of patients with acquired immunodeficiency syndrome (AIDS). The epsilon4 allele of the apolipoprotein E (APOE) gene currently is thought to play a role as a risk factor for the development of HIV dementia. The HIV protein Tat is neurotoxic and binds to the same receptor as apoE, the low-density lipoprotein receptor-related protein (LRP). In this study, we investigated the role apoE plays in Tat toxicity. Synaptosomes from wild-type mice treated with Tat had increased reactive oxygen species (ROS), increased lipid and protein oxidation, and decreased mitochondrial membrane potential. Synaptosomes from APOE-knockout mice also had increased ROS, increased protein oxidation, and decreased mitochondrial membrane potential, but to a significantly lesser degree. Treatment of synaptosomes with heparinase and Tat increased Tat-induced oxidative stress, consistent with the notion of Tat requiring interaction with neuronal membranes to induce oxidative damage. Human lipidated apoE3 greatly protected neurons from Tat-induced toxicity, whereas human lipidated apoE4 showed no protection. We demonstrated that human apoE3 has antioxidant properties against Tat-induced toxicity. Taken together, the data suggest that murine apoE and human apoE4 act similarly and do not protect the cell from Tat-induced toxicity. This would allow excess Tat to remain outside the cell and interact with synaptosomal membranes, leading to oxidative stress and neurotoxicity, which could contribute to dementia associated with HIV. We show that the antioxidant properties of apoE3 greatly outweigh the competition for clearance in deterring Tat-induced oxidative stress.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Aug
pubmed:issn
0360-4012
pubmed:author
pubmed:copyrightInfo
Copyright 2004 Wiley-Liss, Inc.
pubmed:issnType
Print
pubmed:day
15
pubmed:volume
77
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
532-9
pubmed:dateRevised
2007-11-15
pubmed:meshHeading
pubmed-meshheading:15264223-AIDS Dementia Complex, pubmed-meshheading:15264223-Animals, pubmed-meshheading:15264223-Antioxidants, pubmed-meshheading:15264223-Apolipoprotein E3, pubmed-meshheading:15264223-Apolipoprotein E4, pubmed-meshheading:15264223-Apolipoproteins E, pubmed-meshheading:15264223-Cell Membrane, pubmed-meshheading:15264223-Cells, Cultured, pubmed-meshheading:15264223-Gene Products, tat, pubmed-meshheading:15264223-Genetic Predisposition to Disease, pubmed-meshheading:15264223-Humans, pubmed-meshheading:15264223-Lipid Peroxidation, pubmed-meshheading:15264223-Metabolic Clearance Rate, pubmed-meshheading:15264223-Mice, pubmed-meshheading:15264223-Mice, Knockout, pubmed-meshheading:15264223-Neurons, pubmed-meshheading:15264223-Neuroprotective Agents, pubmed-meshheading:15264223-Oxidative Stress, pubmed-meshheading:15264223-Reactive Oxygen Species, pubmed-meshheading:15264223-Synaptosomes, pubmed-meshheading:15264223-tat Gene Products, Human Immunodeficiency Virus
pubmed:year
2004
pubmed:articleTitle
Effects of apolipoprotein E on the human immunodeficiency virus protein Tat in neuronal cultures and synaptosomes.
pubmed:affiliation
Department of Chemistry, University of Kentucky, Lexington, Kentucky 40506, USA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't