15263006

Source:http://linkedlifedata.com/resource/pubmed/id/15263006

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Predicate	Object
rdf:type	pubmed:Citation
lifeskim:mentions	umls-concept:C0007634, umls-concept:C0376209, umls-concept:C0379710, umls-concept:C0449438, umls-concept:C0456081, umls-concept:C0543482, umls-concept:C1709059
pubmed:issue	40
pubmed:dateCreated	2004-9-27
pubmed:abstractText	Morphological change is one of the cardinal features of the senescent phenotype; for example, senescent human diploid cells have a flat large shape. However, the mechanisms underlying such senescence-related morphological alterations have not been well studied. To investigate this situation, we characterized the senescence-dependent changes of cellular structural determinants in terms of their levels and activities. These determinants included integrins, focal adhesion complexes, and small Rho GTPases, and special emphasis was placed on their relationships with caveolin-1 status. We observed that the expression integrin beta(1) and focal adhesion kinase (FAK) were increased and that the phosphorylations of FAK and paxillin, hallmarks of focal adhesion formation, were also increased in senescent human diploid fibroblast cells. Moreover, the Rho GTPases Rac1 and Cdc42 were found to be highly activated in senescent cells. In addition, focal adhesion complexes and Rho GTPases were up-regulated in the caveolin-rich membrane domain in the senescent cells. Activated Rac1 and Cdc42 directly interacted with caveolin-1 in senescent cells. Interestingly, caveolin-1 knock-out senescent cells, achieved by using small interfering RNA and antisense oligonucleotide, showed disrupted focal adhesion formation and actin stress fibers via the inactivation of FAK, which resulted in morphological adjustment to the young cell-like small spindle shape. Based on the results obtained, we propose that caveolin-1 plays an important role in senescence-associated morphological changes by regulating focal adhesion kinase activity and actin stress fiber formation in the senescent cells.
pubmed:language	eng
pubmed:journal	http://linkedlifedata.com/resource/pubmed/journal/2985121R
pubmed:citationSubset	IM
pubmed:chemical	http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD29, http://linkedlifedata.com/resource/pubmed/chemical/CAV1 protein, human, http://linkedlifedata.com/resource/pubmed/chemical/Caveolin 1, http://linkedlifedata.com/resource/pubmed/chemical/Caveolins, http://linkedlifedata.com/resource/pubmed/chemical/Focal Adhesion Kinase 1, http://linkedlifedata.com/resource/pubmed/chemical/Focal Adhesion Protein-Tyrosine..., http://linkedlifedata.com/resource/pubmed/chemical/Integrins, http://linkedlifedata.com/resource/pubmed/chemical/PTK2 protein, human, http://linkedlifedata.com/resource/pubmed/chemical/Protein-Tyrosine Kinases, http://linkedlifedata.com/resource/pubmed/chemical/cdc42 GTP-Binding Protein, http://linkedlifedata.com/resource/pubmed/chemical/rac1 GTP-Binding Protein, http://linkedlifedata.com/resource/pubmed/chemical/rho GTP-Binding Proteins
pubmed:status	MEDLINE
pubmed:month	Oct
pubmed:issn	0021-9258
pubmed:author	pubmed-author:ChoKyung AKA, pubmed-author:JangIk SoonIS, pubmed-author:KimHwang-PhillHP, pubmed-author:KimKyung TaeKT, pubmed-author:LeeJung WeonJW, pubmed-author:OhYoon SinYS, pubmed-author:ParkJi HyeunJH, pubmed-author:ParkSang ChulSC, pubmed-author:RyuSung JinSJ
pubmed:issnType	Print
pubmed:day	1
pubmed:volume	279
pubmed:owner	NLM
pubmed:authorsComplete	Y
pubmed:pagination	42270-8
pubmed:dateRevised	2009-11-19
pubmed:meshHeading	pubmed-meshheading:15263006-Antigens, CD29, pubmed-meshheading:15263006-Caveolin 1, pubmed-meshheading:15263006-Caveolins, pubmed-meshheading:15263006-Cell Aging, pubmed-meshheading:15263006-Cell Size, pubmed-meshheading:15263006-Cells, Cultured, pubmed-meshheading:15263006-Fibroblasts, pubmed-meshheading:15263006-Focal Adhesion Kinase 1, pubmed-meshheading:15263006-Focal Adhesion Protein-Tyrosine Kinases, pubmed-meshheading:15263006-Focal Adhesions, pubmed-meshheading:15263006-Humans, pubmed-meshheading:15263006-Integrins, pubmed-meshheading:15263006-Protein Binding, pubmed-meshheading:15263006-Protein-Tyrosine Kinases, pubmed-meshheading:15263006-Transfection, pubmed-meshheading:15263006-Up-Regulation, pubmed-meshheading:15263006-cdc42 GTP-Binding Protein, pubmed-meshheading:15263006-rac1 GTP-Binding Protein, pubmed-meshheading:15263006-rho GTP-Binding Proteins
pubmed:year	2004
pubmed:articleTitle	Morphological adjustment of senescent cells by modulating caveolin-1 status.
pubmed:affiliation	Department of Biochemistry and Molecular Biology, Aging and Apoptosis Research Center, National University College of Medicine, Seoul, Korea.
pubmed:publicationType	Journal Article, Research Support, Non-U.S. Gov't