Linked Life Data

1525978

Source:http://linkedlifedata.com/resource/pubmed/id/1525978

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
1992-10-22
pubmed:abstractText
Preliminary observations have indicated that both heavy and light chains of monoclonal immunoglobulin exhibit diagnostically useful microheterogeneity patterns when examined with high resolution two-dimensional electrophoresis (2DE) and silver staining (Clin Chem 1990; 36: 993). The present study is a survey of the distribution and types of monoclonal light chain isoforms as observed with 2DE in a group of 68 sera and urine specimens from a cohort of 41 patients with monoclonal gammopathy. The light chain patterns were patient-specific and varied in both the type (kappa, lambda, charge or mass or both) and the number of isoforms present (one to six). A large proportion (31 of 41 cases, 75.6%) exhibited significant microheterogeneity, i.e., distribution of light-chains among two or more isoforms. Charge-based microheterogeneity alone occurred in 17 cases and both charge and mass isoforms were observed in 14 cases. The distribution of charge isoforms of the light chains corresponded qualitatively to the densitometric profiles of agarose gel and immunofixation electrophoresis studies. There was no correlation between heavy chain type and light chain pattern. A carbamylation assay was used to demonstrate that the chemical charge differences between the isoforms were unitary. Ascertainment of this light-chain microheterogeneity has not only proved useful in resolving some diagnostic immunoglobulin typing dilemmas but also is germane to understanding the molecular basis of neoplastic B-cell paraprotein metabolism.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Aug
pubmed:issn
0009-9120
pubmed:author
pubmed:issnType
Print
pubmed:volume
25
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
235-43
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1992
pubmed:articleTitle
Patient-specific microheterogeneity patterns of monoclonal immunoglobulin light chains as revealed by high resolution, two-dimensional electrophoresis.
pubmed:affiliation
Department of Pathology, University of Chicago Pritzker School of Medicine, IL 60637.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, Non-P.H.S., Research Support, Non-U.S. Gov't