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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
3
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pubmed:dateCreated |
2004-7-19
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pubmed:abstractText |
The nuclear pore membrane protein POM121 is specifically degraded during apoptosis by a caspase-3-dependent process enabling early detection of apoptosis in living cells expressing POM121-GFP. Here we further investigated temporal aspects of apoptotic degradation of POM121-GFP. We demonstrate that decreased POM121-GFP fluorescence precedes annexin V-labelling of apoptotic cells. This indicates that degradation of the nuclear pore complex starts prior to redistribution of plasma membrane phosphatidylserine, which serves as a signal for phagocytotic elimination of apoptotic cells. Furthermore, a caspase-resistant GFP-labelled mutant of POM121 resisted degradation even in late apoptosis and was detected in clustered nuclear pores. Thus, it can be concluded that loss of POM121-GFP is a specific sensor of the activation of caspase-3-dependent proteolysis at the nuclear pores.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
May
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pubmed:issn |
1360-8185
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
9
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
363-8
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:15258468-Animals,
pubmed-meshheading:15258468-Annexin A5,
pubmed-meshheading:15258468-Apoptosis,
pubmed-meshheading:15258468-Caspases,
pubmed-meshheading:15258468-Cell Line,
pubmed-meshheading:15258468-Cell Nucleus,
pubmed-meshheading:15258468-Cricetinae,
pubmed-meshheading:15258468-Cricetulus,
pubmed-meshheading:15258468-Enzyme Activation,
pubmed-meshheading:15258468-Hydrolysis,
pubmed-meshheading:15258468-Membrane Glycoproteins,
pubmed-meshheading:15258468-Membrane Proteins,
pubmed-meshheading:15258468-Microscopy, Fluorescence,
pubmed-meshheading:15258468-Mutation,
pubmed-meshheading:15258468-Nuclear Pore,
pubmed-meshheading:15258468-PC12 Cells,
pubmed-meshheading:15258468-Phosphatidylserines,
pubmed-meshheading:15258468-Rats,
pubmed-meshheading:15258468-Recombinant Proteins,
pubmed-meshheading:15258468-Time Factors
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pubmed:year |
2004
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pubmed:articleTitle |
Degradation of GFP-labelled POM121, a non-invasive sensor of nuclear apoptosis, precedes clustering of nuclear pores and externalisation of phosphatidylserine.
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pubmed:affiliation |
Department of Neurochemistry and Neurotoxicology, Stockholm University, SE-106 91 Stockholm, Sweden.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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